Based on various proteins involved in the complement system, Creative Biolabs offers specific assay kits to detect new receptors or targets in various diseases, to assess protein-protein interactions, to quantitate the amount of target analytes or monitor protein expression levels in biological samples.
To better understand complement system activation and its impact on disease, a variety of complement assay kits are available, each tailored to address a different complement activation pathway.
Activation Pathway | Description | Common Test Markers and Components | Research Applications |
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Classical Pathway Assay Kits | Classical pathway assay kits are designed to assess complement activity through activation of this pathway. These kits typically measure activation of key components (e.g., C1q, C3, and C5) as well as production of the terminal complement complex (TCC). Several formats are available, including colorimetric, ELISA-based and flow cytometry-based assays. |
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Alternative Pathway Assay Kits | Alternative pathway assay kits target the activation of alternative complement cascades. These kits typically measure C3b, C3 converting enzyme production and amplification loop activity. The assays typically utilize key components such as Factor B, Factor D, and prolysin, which play a critical role in the alternative pathway. In addition, detection of surface C3b deposition can be an indicator of pathway activation. |
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Lectin Pathway Assay Kits | Lectin pathway assay kits are designed to detect and quantify activation of the lectin pathway. These assays typically focus on measuring C3 and C4 activation, C3b deposition, and C3 convertase formation. These kits also assess the involvement of lectin receptors such as MBL and fibronectin and their associated MASPs. |
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Complement function assay kits have become an indispensable tool for life scientists. These kits provide a powerful platform for quantitative and qualitative analysis of complement activity and individual components, ensuring that experiments are precise, reproducible and of high throughput. Complement assay kits are available in a variety of formats, each optimized for different applications.
The hemolytic assay is the gold standard for assessing the functional activity of the complement system, providing key insights into its overall efficiency. Complement hemolysis assays are particularly well suited for assessing classical, alternative or lectin pathway activity, providing reliable and quantitative measurements.
Principle
The assay measures complement-mediated lysis of sensitized red blood cells. Quantitative hemolytic activity kits typically include:
Applications
ELISA techniques enable precise detection and quantification of individual complement components such as C3, C5 or their activated fragments (e.g. C3a, C5a). These assays are particularly useful for analyzing the contribution of specific proteins to complement function.
Principle
These kits typically include:
Applications
The complexity of the complement cascade requires assays that can evaluate multiple proteins and pathways simultaneously. Multiplexed assays fulfill this requirement, allowing researchers to gain a comprehensive understanding of the system with minimal sample size.
Features
Principle
Applications
Why are Complement Function Assay Kits Essential?
These kits have become essential tools for immunology, drug development and translational biology researchers. Key benefits include:
For detailed specifications or to learn about the latest assay kits for your research needs, contact Creative Biolabs - your trusted partner for advanced complement system research tools.
Tailored solutions to meet the unique needs of specialized research projects. Our customization options include:
Options | Specific Description |
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Targeting kits | Customized assays can be developed to target new or understudied complement components, enabling exploratory research in emerging areas such as complement-driven diseases or therapeutic interventions. |
Advanced assay platforms | Integration with cutting-edge detection technologies such as fluorescence resonance energy transfer (FRET) or bioluminescence for enhanced sensitivity. |
Adaptation to alternative readout formats (e.g. colorimetric, chemiluminescent). | |
Specialized applications | Develop assays to study complement-mediated cell death, immune complex clearance, or complement-related biomarkers. |
Optimize for specific sample types, including serum, plasma or cell culture supernatants. | |
Scalability for drug development | High-throughput screening formats for the discovery of inhibitors. |
Lot-to-lot consistency testing during manufacturing. |
Kit Components
Each of our complement assay kits is carefully constructed using high-quality materials to ensure reliable and reproducible results. Key components include proprietary complement proteins, pathway-specific reagents, optimized assay kits, standardized controls, and validated buffers and solutions.
Assay Parameters
The validity of a complement assay kit depends on its ability to provide consistent, sensitive and reproducible results in a variety of applications. Key assay parameters include:
Parameters | Specific Descriptions |
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Sensitivity |
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Dynamic Range |
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Sample Compatibility |
Compatible with a wide range of sample types including:
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Time to Results |
A rapid test protocol designed for convenience:
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Storage |
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Ready to Get a Quote?
For more information on the range of complement assay kits available, or to request a customized solution for your research needs, please contact Creative Biolabs.
Explains the strict criteria that ensure our kits provide accurate results even in complex samples.
Emphasizes easy-to-understand procedures for new and experienced researchers.
Emphasizes compatibility with automated systems for large laboratories or drug development workflows.
Outlines the rigorous quality checks performed to ensure the reliability and reproducibility of batches.
Reinforces Creative Biolabs' position as an expert in complement-related tools.
Emphasizes the breadth and specificity of our product offerings, addressing the needs of each complement pathway.
Demonstrates the strong quality assurance and customer support that Creative Biolabs provides to enhance the user experience and laboratory success.
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Creative Biolabs offers a range of high-quality products for complement activity and function assays. View our product list to find your interested products.
Complement Function Analysis Protocols
We briefly summarize the complement-related assay protocols. Find complement-related assay protocols by clicking on the following it
Our complement assay kits are designed for different components of the complement cascade. For optimal selection, consider your target, sample type, and desired detection method. We recommend reviewing the detailed specifications for each kit and consulting our technical support team who can guide you based on your research objectives, sample availability, and required sensitivity range. We also offer validation data for different sample types to help inform your decision.
When encountering inconsistent results, first check sample handling and storage conditions, as complement proteins are sensitive to temperature and freeze-thaw cycles. Verify proper sample collection protocols, including appropriate anticoagulants and processing times. Common issues include improper temperature control during assay execution, incorrect dilution ratios, or matrix effects. Our technical support team can provide a comprehensive troubleshooting guide and assist with protocol optimization. We also recommend running appropriate controls and standard curves with each assay to ensure validity.
Yes, our complement assay kits are compatible with most automated laboratory platforms. We provide detailed protocols optimized for common automated systems, including specific parameters for liquid handling and incubation steps. Our technical team can assist with protocol adaptation for your specific automation setup. We also offer larger kit sizes and bulk reagent options suitable for high-throughput screening applications. Additionally, we can provide validation support and instrument-specific recommendations to ensure optimal performance in automated workflows.
We offer customization options for specific research needs, including bulk ordering, custom kit configurations, and specialized formulations. Our team can work with you to modify kit components, detection methods, or assay formats to meet your requirements. Custom formulation requests undergo feasibility assessment and validation testing to ensure they meet our quality standards while satisfying your specific research needs.
Our complement assay kits undergo extensive cross-reactivity testing with samples from various species including human, mouse, rat, and other commonly used research models. Each kit's datasheet specifies validated species and includes expected value ranges for different sample types. For species not listed in our standard validation panel, we can provide additional testing data or assist with pilot studies to confirm compatibility.
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