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Assay Kits

Assay Kits

Based on various proteins involved in the complement system, Creative Biolabs offers specific assay kits to detect new receptors or targets in various diseases, to assess protein-protein interactions, to quantitate the amount of target analytes or monitor protein expression levels in biological samples.

Complement Assay Kits by Pathway Hemolytic Assay Kits ELISA-based Functional Assay Kits Multiplexed Assay Kits Customization of Complement Assay Kits Technical Specifications of Product Why Choose Creative Biolabs’ Complement Assay Kits? FAQs Popular in Collection Related Products & Services

Complement Assay Kits by Pathway

To better understand complement system activation and its impact on disease, a variety of complement assay kits are available, each tailored to address a different complement activation pathway.

Activation Pathway Description Common Test Markers and Components Research Applications
Classical Pathway Assay Kits Classical pathway assay kits are designed to assess complement activity through activation of this pathway. These kits typically measure activation of key components (e.g., C1q, C3, and C5) as well as production of the terminal complement complex (TCC). Several formats are available, including colorimetric, ELISA-based and flow cytometry-based assays.
  • C1q: key promoter of the classical pathway, recognizes the Fc region of the antibody.
  • C3: central component of all complement pathways, its cleavage products (C3a and C3b) are hallmarks of activation.
  • C5: involved in the formation of the membrane attack complex (MAC).
  • TCC: complex formed during the late stages of complement activation and can be used as a marker of complement activation.
  • Studying antibody-dependent immune responses
  • Understanding complement activation in the context of disease
Alternative Pathway Assay Kits Alternative pathway assay kits target the activation of alternative complement cascades. These kits typically measure C3b, C3 converting enzyme production and amplification loop activity. The assays typically utilize key components such as Factor B, Factor D, and prolysin, which play a critical role in the alternative pathway. In addition, detection of surface C3b deposition can be an indicator of pathway activation.
  • Factor B: a key component of the C3 convertase complex in the alternative pathway.
  • Factor D: an enzyme that cleaves factor B to form C3 convertase.
  • Properdin: stabilizers of the alternative pathway C3 convertase complex that contribute to its amplification.
  • Autoimmunity research
  • Innate immunity study
Lectin Pathway Assay Kits Lectin pathway assay kits are designed to detect and quantify activation of the lectin pathway. These assays typically focus on measuring C3 and C4 activation, C3b deposition, and C3 convertase formation. These kits also assess the involvement of lectin receptors such as MBL and fibronectin and their associated MASPs.
  • MBL: key promoter of the lectin pathway, recognizes carbohydrate patterns on the surface of pathogens.
  • Ficolins: proteins that, like MBL, bind to carbohydrate structures and initiate complement activation.
  • MASP: enzyme that cleaves C4 and C2 to form C3 converting enzyme.
  • Infection response
  • Pathogen-host interactions

Complement function assay kits have become an indispensable tool for life scientists. These kits provide a powerful platform for quantitative and qualitative analysis of complement activity and individual components, ensuring that experiments are precise, reproducible and of high throughput. Complement assay kits are available in a variety of formats, each optimized for different applications.

Hemolytic Assay Kits

The hemolytic assay is the gold standard for assessing the functional activity of the complement system, providing key insights into its overall efficiency. Complement hemolysis assays are particularly well suited for assessing classical, alternative or lectin pathway activity, providing reliable and quantitative measurements.

Principle

The assay measures complement-mediated lysis of sensitized red blood cells. Quantitative hemolytic activity kits typically include:

  • Sensitized erythrocytes (sheep, rabbit, or other sources).
  • Buffer solutions optimized for each complement pathway.
  • Calibrators for precise quantification of activity.

Applications

  • Assessing complement defects or pathway dysregulation.
  • To study drug-induced complement activation.
  • Test complement inhibitors or therapeutics targeting specific pathways.

ELISA-based Functional Assay Kits

ELISA techniques enable precise detection and quantification of individual complement components such as C3, C5 or their activated fragments (e.g. C3a, C5a). These assays are particularly useful for analyzing the contribution of specific proteins to complement function.

  • High sensitivity and specificity: capture and detection antibodies ensure minimal cross-reactivity.
  • Broad sample compatibility: Suitable for plasma, serum, cell culture supernatants and other biological matrices.
  • Quantitative data: useful for kinetic studies and dose-response analysis.

Principle

These kits typically include:

  • Plates pre-coated with high affinity capture antibodies.
  • Calibration standards for absolute quantitation.
  • Matrix solutions for reliable signal detection.

Applications

  • Analyze complement activation in disease models, including autoimmune diseases, cancer, and infectious diseases.
  • Evaluate the efficacy of complement-targeted drugs.
  • Study complement protein expression in transgenic animal models.

Multiplexed Assay Kits

The complexity of the complement cascade requires assays that can evaluate multiple proteins and pathways simultaneously. Multiplexed assays fulfill this requirement, allowing researchers to gain a comprehensive understanding of the system with minimal sample size.

Features

  • Reduced sample size: ideal for experiments with limited material, such as rare patient samples.
  • High throughput: analyze more than 10 complement factors simultaneously in a single run.
  • Cost and time efficient: consolidate experiments, and reduce reagent consumption and labor.

Principle

  • Bead-based systems: utilize fluorescent or magnetic beads conjugated to specific antibodies to detect various complement factors in parallel.
  • Microarray platform: utilizes spatially resolved capture antibody arrays for simultaneous protein quantification.
  • Advanced detection system: rapid readout utilizing flow cytometry or imaging.

Applications

  • Comprehensive analysis of complement activation across pathways.
  • Study complement interactions with other immune mechanisms.
  • High-throughput drug screening for complement modulators.

Why are Complement Function Assay Kits Essential?

These kits have become essential tools for immunology, drug development and translational biology researchers. Key benefits include:

  • Enhanced precision: standardized protocols ensure consistent and reproducible results.
  • Versatility: kits are suitable for use in a variety of models, including in vitro cell cultures, in vivo disease models, and ex vivo human samples.
  • Scalability: from single-protein ELISAs to multiplexed platforms, they meet diverse research.

For detailed specifications or to learn about the latest assay kits for your research needs, contact Creative Biolabs - your trusted partner for advanced complement system research tools.

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Customization of Complement Assay Kits

Tailored solutions to meet the unique needs of specialized research projects. Our customization options include:

Options Specific Description
Targeting kits Customized assays can be developed to target new or understudied complement components, enabling exploratory research in emerging areas such as complement-driven diseases or therapeutic interventions.
Advanced assay platforms Integration with cutting-edge detection technologies such as fluorescence resonance energy transfer (FRET) or bioluminescence for enhanced sensitivity.
Adaptation to alternative readout formats (e.g. colorimetric, chemiluminescent).
Specialized applications Develop assays to study complement-mediated cell death, immune complex clearance, or complement-related biomarkers.
Optimize for specific sample types, including serum, plasma or cell culture supernatants.
Scalability for drug development High-throughput screening formats for the discovery of inhibitors.
Lot-to-lot consistency testing during manufacturing.

Technical Specifications of Product

Kit Components

Each of our complement assay kits is carefully constructed using high-quality materials to ensure reliable and reproducible results. Key components include proprietary complement proteins, pathway-specific reagents, optimized assay kits, standardized controls, and validated buffers and solutions.

  • 1

    Proprietary complement proteins

    • Purified, recombinant or natural proteins from known sources.
    • Essential components of the classical, alternative and lectin pathways.
    • Proteins are lyophilized or supplied in ready-to-use liquid form to maintain biological activity and stability.
  • 2

    Pathway-specific activators and inhibitors

    • Specific activators such as immunocomplexes, yeast glycans or mannan-coated plates for stimulation of the classical, alternative or lectin pathways, respectively.
    • Inhibitors such as Compstatin or small molecule blockers are included for studying regulatory mechanisms or validating results.
    • All reagents are rigorously tested to ensure selectivity and consistent pathway activation or inhibition.
  • 3

    Optimized detection reagents

    • Highly sensitive substrates (e.g., chromogenic, fluorescent, or luminescent) compatible with a wide range of detection platforms.
    • Antibody-based tools, including enzyme-linked or fluorescently labeled antibodies, target key complement fragments.
    • Reagents are optimized for excellent signal-to-noise and minimal cross-reactivity.
  • 4

    Standardized controls

    • Positive control: pre-activated complement sample to validate assay functionality.
    • Negative control: heat-inactivated sample to exclude non-specific activity.
    • Internal standard: reference material used to accurately quantify complement activity.
  • 5

    Validated buffers and solutions

    • Buffers designed to maintain complement activity during the assay.
    • Pre-tested solutions for optimal pH, ionic strength and stability.
    • Ready-to-use wash buffers, sealers, and dilution solutions simplify workflow.

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Assay Parameters

The validity of a complement assay kit depends on its ability to provide consistent, sensitive and reproducible results in a variety of applications. Key assay parameters include:

Parameters Specific Descriptions
Sensitivity
  • Designed to detect small changes in complement activity with detection limits in the picomolar or nanomolar range.
  • Suitable for low abundance targets in complex biological matrices such as serum, plasma or cerebrospinal fluid.
Dynamic Range
  • Wide dynamic range allows quantification of complement activity over a broad range from basal to hyperactive states.
  • Linear response curves provide reliable data interpretation and accurate quantification.
Sample Compatibility Compatible with a wide range of sample types including:
  • Human and animal serum/plasma
  • Cell culture supernatants for in vitro studies
  • Tissue homogenates
Time to Results A rapid test protocol designed for convenience:
  • Total run time: 1-4 hours, depending on assay type.
  • Simplified steps to minimize hands-on time.
Storage
  • Long shelf life, components remain stable at -20°C or 4°C, depending on the reagent.
  • Lyophilized reagents can remain active for up to 24 months when stored under recommended conditions.

Ready to Get a Quote?

For more information on the range of complement assay kits available, or to request a customized solution for your research needs, please contact Creative Biolabs.

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Why Choose Creative Biolabs' Complement Assay Kits?

  • Accuracy and Sensitivity

Explains the strict criteria that ensure our kits provide accurate results even in complex samples.

  • User-Friendliness

Emphasizes easy-to-understand procedures for new and experienced researchers.

  • High-Throughput Capability

Emphasizes compatibility with automated systems for large laboratories or drug development workflows.

  • Quality Control

Outlines the rigorous quality checks performed to ensure the reliability and reproducibility of batches.

  • Expertise in Complement Biology

Reinforces Creative Biolabs' position as an expert in complement-related tools.

  • Comprehensive Product Portfolio

Emphasizes the breadth and specificity of our product offerings, addressing the needs of each complement pathway.

  • Unrivaled Quality and Service

Demonstrates the strong quality assurance and customer support that Creative Biolabs provides to enhance the user experience and laboratory success.

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Creative Biolabs offers a range of high-quality products for complement activity and function assays. View our product list to find your interested products.

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Complement Function Analysis Protocols

We briefly summarize the complement-related assay protocols. Find complement-related assay protocols by clicking on the following it

FAQs

How do I know which complement assay kit is most suitable for my specific research application?

Our complement assay kits are designed for different components of the complement cascade. For optimal selection, consider your target, sample type, and desired detection method. We recommend reviewing the detailed specifications for each kit and consulting our technical support team who can guide you based on your research objectives, sample availability, and required sensitivity range. We also offer validation data for different sample types to help inform your decision.

If I get inconsistent results with my complement assay, what are the most common troubleshooting steps I should take?

When encountering inconsistent results, first check sample handling and storage conditions, as complement proteins are sensitive to temperature and freeze-thaw cycles. Verify proper sample collection protocols, including appropriate anticoagulants and processing times. Common issues include improper temperature control during assay execution, incorrect dilution ratios, or matrix effects. Our technical support team can provide a comprehensive troubleshooting guide and assist with protocol optimization. We also recommend running appropriate controls and standard curves with each assay to ensure validity.

Can your complement assay kits be used with automated laboratory systems?

Yes, our complement assay kits are compatible with most automated laboratory platforms. We provide detailed protocols optimized for common automated systems, including specific parameters for liquid handling and incubation steps. Our technical team can assist with protocol adaptation for your specific automation setup. We also offer larger kit sizes and bulk reagent options suitable for high-throughput screening applications. Additionally, we can provide validation support and instrument-specific recommendations to ensure optimal performance in automated workflows.

Do you provide custom formulations or bulk ordering options for complement assay kits?

We offer customization options for specific research needs, including bulk ordering, custom kit configurations, and specialized formulations. Our team can work with you to modify kit components, detection methods, or assay formats to meet your requirements. Custom formulation requests undergo feasibility assessment and validation testing to ensure they meet our quality standards while satisfying your specific research needs.

Are your complement assay kits validated for use with different species samples?

Our complement assay kits undergo extensive cross-reactivity testing with samples from various species including human, mouse, rat, and other commonly used research models. Each kit's datasheet specifies validated species and includes expected value ranges for different sample types. For species not listed in our standard validation panel, we can provide additional testing data or assist with pilot studies to confirm compatibility.

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