PathSpecific™ Caspase-6 Activity Assay Kit, Colorimetric (CAT#: ZL-1122-WR35)
| Overview | |
|---|---|
| Description | The kit is used to measure the enzyme activity of caspase 6 in cell lysate, tissue extracts, and biological samples. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Caspase 6 protease in biological samples. |
| Application Notes | This kit is a quick and easy way to test for caspase activity that targets the VEID sequence. |
| Molecular Mechanism of Action | The assay relies on the enzymatic breakdown of the labeled substance Ac-VEID-pNA (N-Acetyl-Val-Glu-Ile-Asp-p-nitroanilide) by Caspase-6, which subsequently releases the pNA (p-nitroaniline) component. The absortion peak of the freed pNA is at 405 nm (εmM = 10.5) and its concentration can be quantified by measuring the absorption at 405 nm or by comparing it to a standard calibration curve prepared using the pNA Dye Standard. This can be accomplished using a microplate reader or spectrophotometer. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 50 tests;100 tests |
| Components | The kit is supplied with an assay kit including caspase lysis buffer, caspase assay buffer, Ac-VEID-pNA substrate, pNA dye standard, and caspase inhibitor Z-VAD-FMK. |
| Target | Caspase 6 |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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