PathSpecific™ Human Cathepsin C ELISA Kit, Colorimetric (CAT#: ZL-1122-WR14)
| Overview | |
|---|---|
| Description | The kit is designed to quantitatively measure the cathepsin C activity in cell culture supernatant, plasma, heparinized plasma, and serum samples. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin C protease in biological samples. |
| Application Notes | The kit is a quantitative assay used to measure the levels of Human Cathepsin C in serum and plasma samples, providing valuable insights into its activity and potential implications in various diseases. |
| Molecular Mechanism of Action | This assay detects human Cathepsin C using a specific antibody coated on a 96-well plate. Samples are added to the wells, and Cathepsin C present in the sample binds to the immobilized antibody. After washing away unbound components, a biotinylated anti-Human Cathepsin C antibody is added. Following another wash, an HRP-conjugated streptavidin is added. After another wash, a TMB substrate is added and the intensity of the resulting color is measured at 450 nm. |
| Assay Measures | ELISA method |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with an assay kit including 100X HRP-streptavidin concentrate, 20X wash buffer, 5X assay diluent, biotinylated anti-human cathepsin C antibody, anti-Human cathepsin C coated microplate, human cathepsin C standard protein (Lyophilized), stop solution, and TMB one-step substrate reagent. |
| Target | Cathepsin C |
| Species Reactivity | Human |
| Sensitivity | 2 ng/ml |
| Format | Supplied with ELISA assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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