Custom LNP Formulation Service for Circular RNAs
In recent years, lipid nanoparticles (LNPs) have gradually emerged as promising tools in the field of gene therapy due to their advantages in potency, payload, and design flexibility. As a senior expert in this field, Creative Biolabs is committed to providing custom LNP encapsulation service for circular RNAs (circRNAs) delivery.
Overview of LNPs
LNPs, one of the novel potential delivery tools in the 100-150 nm range, are an essential alternative to polymers system. LNPs are lipophile substances that have been shown to be effective in delivering nucleic acid therapeutic products. The delivery system uses a biocompatible lipid material as a carrier, capable of dissolving, wrapping, adsorbing, and attaching drugs or other biologically active substances. Compared with other delivery systems, the LNP system has many advantages, including enhanced drug absorption, altered drug toxicity, enhanced drug stability, and controlled drug release. Therefore, LNPs play an increasingly important role in the delivery of gene medicines, antineoplastic drugs, proteins and peptides.
Fig.1 LNPs for RNA delivery.¹
Applications
Currently, LNPs are increasingly being considered a viable drug delivery system. In addition to therapeutics, LNPs have been investigated in various fields, such as medical imaging, cosmetics and agriculture.
- Drug delivery
For the delivery of poorly soluble drugs, this system provides an attractive method. The system is suitable for the delivery of a wide variety of therapeutic agents, including both hydrophilic and hydrophobic molecules. In addition, this system can be targeted to specific sites, which effectively reduces unwanted side effects, making it ideal for targeted therapy.
- Medical imaging
LNPs have been used for medical diagnostics. For example, LNPs are involved in the transfer and localization of fluorescent labels in fluorescence imaging. Furthermore, LNPs may be further modified for the purpose of targeting certain cell types.
- Vaccines delivery
Currently, LNPs have proven to be an effective strategy for delivering new vaccines. Notably, several common mRNA vaccines are encapsulated in LNPs. This delivery system improves the safety, efficiency and shelf life of mRNA delivery in vivo.
- Peptide and nucleic acid therapeutics
The ability to develop nucleic acid and peptide therapeutics is a key feature of LNP technology. Peptide and nucleic acid therapies have been rarely used in medical therapy due to their poor stability and easy degradability. LNPs offer new insights into the delivery of peptides and nucleic acids, as LNP encapsulation allows the contents to be protected from degradation.
Custom LNP Formulation Service for Circular RNAs
With our advanced circRNAs synthesis platform and complete LNP system, Creative Biolabs focuses on providing customized LNP encapsulation of circRNA services for in vitro and in vivo delivery of circRNAs. Furthermore, to ensure efficient delivery of circRNA-LNP complexes, the particle size, zeta potential (ZP), concentration, polydispersibility index (PDI) and encapsulation efficiency of the LNPs were determined.
Fig.2 CircRNA-LNP vaccine.²
Our experienced scientists will work with you to customize a reasonable solution to promote the breakthrough progress of your gene therapy project. For more information and a detailed quotation, please contact us contact us.
References
- Żak, Magdalena M., and Lior Zangi. "Lipid nanoparticles for organ-specific mRNA therapeutic delivery." Pharmaceutics 13.10 (2021): 1675.
- Li, Hongjian, et al. "Circular RNA cancer vaccines drive immunity in hard-to-treat malignancies." Theranostics 12.14 (2022): 6422.
