Nucleic Acid Anaylsis
Nucleic acids, such as DNA and RNA, are composed of oligonucleotides, which are short (oligo-) polymers of nucleotides, serving as their basic subunits. They are made up of 13 to 25 nucleotides and have many uses from research to disease diagnosis and therapeutics. The most common applications are antisense oligonucleotides (ASO), nucleic acid aptamers, allele-specific oligonucleotides (ASO), small interfering RNA (siRNA), and circular RNAs (circRNAs). These nucleic acid products have a wide range of applications in vitro and in vivo and even clinically. Precise measurement of nucleic acid identity, concentration, safety, purity, potency, and integrity is paramount, serving as the foundation for every molecular biology experiment. As a well-known biotechnological company, Creative Biolabs provides full service for nucleic acid analysis, including:
Identifying nucleic acids involves determining the type of nucleic acid (ssDNA, dsDNA, ssRNA, dsRNA, circRNA), its sequence, and any modifications or variations present.
Evaluating the concentration of nucleic acids is essential to guarantee the precision and dependability of subsequent applications.
Nucleic acid safety assessment provides an overview of the identification of impurities in nucleic acids and the potential risks associated with using nucleic acids in various applications.
The potency of nucleic acid is the effectiveness or functional activity of nucleic acid samples. Depending on the type of nucleic acid and its intended use, potency can be evaluated from various perspectives, both in vitro and in vivo.
Nucl eic acid purity refers to the degree to which a nucleic acid sample is free from contaminants or impurities, such as other nucleic acids, proteins, or chemicals. Assessing nucleic acid purity is essential for ensuring the safety and effectiveness of nucleic acid drug development. For example, in RNA interference (RNAi) and mRNA therapies, high-purity siRNA and mRNA improve drug specificity and potency, minimizing off-target effects and adverse reactions. Assessing the purity of nucleic acids is crucial for ensuring the accuracy and reliability of downstream applications.
Tab.1 Methods for nucleic acids analysis.
| Items | Methods | |
|---|---|---|
| Identity of Nucleic Acid | MS, LC-MS-MS, NGS, and the third generation sequencing. | |
| Concentration of Nucleic Acid | UV absorbance, Fluorometric methods, Gel electrophoresis, qPCR. | |
| Safety of Nucleic Acid | Human Source Contamination (HSC) | Facilitating precise identification of the type and extent of exogenous contamination through probe synthesis. |
| Endotoxin | The Limulus Amebocyte Lysate (LAL) test. | |
| Microorganisms | The nucleic acid amplification techniques (NAT), specifically quantitative PCR using Taqman fluorescent probes. | |
| Toxicity | Cell viability assays, gene expression analysis, and histopathological examination. | |
| Immunogenicity | Using lymphocyte proliferation assay in vitro, and white blood cell interleukin determination, lymphocyte expression analysis in vivo. | |
| Potency of Nucleic Acid | In vitro Functional Assays | qPCR or Western blotting. |
| In vivo Biological Activity | Western blotting for protein level detection and protein function measurement. | |
| Structural Integrity and Stability | Gel electrophoresis, UV spectrophotometry, or capillary electrophoresis. | |
| Specificity and Off-Target Effects: | RNA-seq, ChIP-seq, or CLIP-seq for specificity, and computational algorithms or experimental approaches for off-target. | |
| Purity of Nucleic Acid | HPLC, capillary electrophoresis (CE), or mass spectrometry (MS). | |
SERVICES
Creative Biolabs is dedicated to providing top-notch nucleic acid analysis services globally. Our seasoned team of nucleic acid synthesis specialists offers expert technical assistance. For comprehensive details, don't hesitate to reach out and request a quote. We'll respond within 24 hours and tailor an optimal approach for your project.
