Regulated Adenovirus Vector Construction
The virus represents an appealing tool for therapeutic gene transfer because of its high transfection efficiency in a wide range of human cell types. Approximately 70% of the vectors used in gene therapies are performed by viral-based delivery systems. As it belongs to a pathogenic agent, the virus needs to be attenuated to be safely applied in clinical applications. To this end, Creative Biolabs has designed virus-derived vectors that stem from different viral classes, including adenovirus, adeno-associated virus (AAV), lentiviruses, and retroviruses. Here, we offer a multitude of regulated adenovirus vector construction services to achieve gene transfer properly, particularly specific cell-targeted constructions, specific cancer-targeted constructions, and inducible constructions.
Regulated Modification
Adenovirus is a double-stranded DNA virus and is a member of Group I family in Baltimore classification. It is a non-enveloped icosahedral virus which carries a medium-sized genome of 35 kb, harboring numerous essential genes that determine its biological activities and distinguishable serotypes. So far, there're seven species of adenoviruses (A to G), which can be further divided into 57 serotypes (HAdV-1 to 57). To generate non-replicative yet infective adenoviral vectors, helper cells are required to be transfected with vectors and express key proteins for efficient vector production. Up to now, HEK293T cell lines are found the most prominent to work as helper cells. They're specific lab-adapted immortalized cells and can be easily transfected with vector-packaging plasmids, showing robust protein output and ideal titered adenoviral vectors. Recombinant adenoviral vectors are versatile, easily-regulated systems for gene delivery and expression in mammalian cells.
Figure 1. Schematic diagram of the adenoviral vector genome. (Hu, 2016)
Regulated Adenovirus Vector Construction Services
Adenoviral vectors for gene therapy can be significantly enhanced by targeting vectors to specific cell types or disease classifications. This needs the complete elimination of native adenovirus tropism and the introduction of a new binding affinity in viral capsids. Meanwhile, adenoviruses can be altered to selectively infect or replicate in cancer cells. To further improve targeting ability, cell-killing capacity and hiding talent of the virus from host immunity, we focus on the modifications of adenoviral E1 region and the systems allowing for genome-wide modification.
High transduction efficiency and short-term gene expression make adenoviral vector a preferred instrument for in vivo gene delivery. Creative Biolabs has developed a series of technologies to improve adenoviral packaging protocols in terms of purity, titer, viability, and consistency. Our regulated adenovirus vector construction services include but not limited to the following items.
Specific Cell-targeted Modifications
Specific Cancer-targeted Modifications
Inducible Modifications
Features
- High-efficiency rate of host cell infection
- Rapid and high gene expression
- Characterized adenovirus vector systems
- Testing adenoviral vectors in animal disease models
Due to the basic progress in elucidating molecular mechanisms of human diseases and the arrival of post-genomic time, a number of cellular targets and therapeutic genes are available for gene therapy in clinical trials. As a famous biological company in the world, Creative Biolabs is committed to developing gene delivery vectors with high efficiency through target cell selectivity, who has already established the most widely used and regulated adenoviral vector systems. Based on our high reputation and professional services, a growing base of clients come back to us for more adenoviral vector cloning and packaging projects. For more information, please feel free to contact us.
Reference
- Hu, S. (2016). Pseudotyping of lentiviral vector with novel vesiculovirus envelope glycoproteins derived from Chandipura and Piry viruses. Virology. 1-163.
