Mesenchymal Stem Cell
Stem cells can be used as delivery vehicles for regenerative medicine application due to the ability of mobilizing, homing and targeting into inflammatory sites. Owing to the remarkable homing capabilities, immune-modulatory capabilities, differentiation potential, secrete trophic factors and low immunogenicity, Mesenchymal stem cell (MSC) is used in clinical trials, including diabetes, hemophilia A, myocardial infarction, stroke, rheumatoid arthritis, asthma, allergic rhinitis, bone defects, Crohn's disease and lung injury. With years of experience on stem cell development, Creative Biolabs provides various high-quality MSCs for clients worldwide in order to meet a variety of preclinical investigation demands.
MSC as Vehicles for Delivering Therapeutic Genes
MSC can be genetically engineered to introduce beneficial genes to substitute the abnormal gene function at the tissue and organ level. By using gene therapy to design MSCs to increase the natural production of specific desired proteins or to enable them to express proteins outside their natural libraries, the spectrum of diseases in which MSCs can provide therapeutic benefits is possible to be greatly broadened. Here, we offer both viral transduction methods containing adenovirus, Adeno-associated virus and lentiviruses, and non-viral transfection methods , including electroporation, liposomes, polymers, dendrimers, inorganic nanoparticles and magnetic nanoparticles (MNPs) to efficiently produce a wide range of secreted protein products. We aim to provide various types of gene-modified MSCs to enhance the natural abilities to mediate repair within various tissues for the gene therapy research.
MSC as Anti-cancer Gene Delivery Vehicles
MSC has the enormous potential as gene delivery vehicles for the treatment of cancer. MSC can selectively target to sites of injury or inflammation, then mediate repair in the injured tissues by engrafting and generating tissue-specific cells, and blunt the inflammatory response through the release of nutrient factors, as well as activate the endogenous repair mechanisms of tissues to promote healing. MSC not only applies to solid tumors, but also has great therapeutic value in the treatment of glioblastoma multiforme (GBM) and lung metastases arising from both breast cancer and melanoma.
Fig.1 Strategies for ex vivo gene delivery by MSCs
Advantages
- Widely distributed throughout the body
- Differentiate into a wide array of functional cell types both in vitro and in vivo
- Easy to isolate and maintain the potential in culture
- Homing to damaged tissues, solid tumors and metastases after in vivo administration
- Significant immunomodulatory and anti-inflammatory effects
Creative Biolabs provides rigorous and advanced purification methods for multiple MSCs isolated from patient tissues, and culture and expansion, as well as efficient transplantation. Our platform of stem cells can contribute to the gene therapy of repairing tissue and organ in the case of degenerative, ischaemic or inflammatory processes. Creative Biolabs has years of experience providing stem cells services and our outstanding scientists will work with you in developing your research.
Reference
- Porada, C.; et al. (2013). Mesenchymal stem cells as gene delivery vehicles. In Gene Therapy-Tools and Potential Applications. InTech.
