Adenovirus Vector Titration

Adenovirus vectors titer represents the quantities and infectivity of the virus vector particles. Adenovirus vector characterization by titration assay is helpful for further applications of adenovirus vectors. At Creative Biolabs, cutting-edge gene therapy platforms have been utilized to provide convenient adenovirus vector titration services for basic research and preclinical applications.

Adenovirus Vectors Titer

Adenoviruses vectors are a class of widely used gene transfer systems constructed by human adenovirus (especially adenovirus serotype 5) by deleting certain specific gene sequences. Actually, adenovirus has attracted numerous attention as an excellent gene delivery vector primarily due to its advantages (high-efficiency in infection of dividing or non-dividing cells and ease of large-scale production, purification, and concentration). After the development of first-generation, second-generation, and third-generation, adenoviral vectors are now extensively applied for gene therapy, vaccine delivery, and cancer immunotherapy.

Adenovirus vectors titer is a parameter commonly utilized to represent the concentration or the number of infectious adenovirus vectors particles. Determination of adenovirus vectors titer is of great significance since it is a measure of viral vector effectiveness, which gives guidance to chose the optimum concentration of adenovirus vector transfection.

Figure 1. Methods of preparation of different types of adenoviral vectors. (Singh, 2018)

Adenovirus Vector Titration Services

As mentioned before, adenovirus vectors titer is an important indicator of viral vector concentration and efficiency. Adenovirus vector titration not only helps to avoid the low success rate of transfection caused by low titer, but also reduces the waste of adenoviral vectors caused by high titer, and even prevents host cells death due to excessive viral vectors infection. Therefore, Creative Biolabs has developed a series of adenovirus vector titration assays to characterize the recombinant adenovirus vectors. Our adenovirus vector titration strategies mainly include but not limited to:

1. 50% tissue culture infective dose (TCID50): determining the adenoviral vectors titration by measuring the adenovirus that can cause cytopathic effect in half of the cell culture plates or test tubes.
2. Serial dilution method: using serially-diluted adenovirus vectors to infect 293A cells, counting the number of transduced cells via the detection of adenovirus-specific hexon protein based on immunocytochemistry.
3. Plaque assay for purified adenovirus vector titration: single-layer HEK293 cells are infected with a series of adenovirus vectors dilutions. The adenovirus multiplied in the infected cells and infected the adjacent cells, producing cytotoxic effects and eventually plaque formation.
4. Other determination assays: focus forming assay, bicinchoninic acid assay, etc.

Adenovirus

With the first-class expertise of viral vector technology, Creative Biolabs provides a diversity of adenovirus and adenoviral vector titration services for the application of gene therapy and vaccine development. Additionally, based on clients' specific demands, we also offer other various adenovirus vector services, such as:

• Recombinant adenovirus construction
• Adenovirus vector purification
• Adenovirus-based cDNA expression libraries generation
• Upstream bioprocess development for adenovirus vector
• Downstream bioprocess development for adenovirus vector
• Helper-dependent adenoviral vectors
• Adenoviral vector-based vaccine development
• Development of adenoviral vector as immune stimulant
• Adenoviral vector-based suicide gene therapy development
• Adenoviral vector design for RNAi delivery

If you are interested, please directly contact us or send us an inquiry, we can customize our offering to meet your specific project needs.

Reference

1. Singh, S.; et al. (2018). Adenoviral Vector-Based Vaccines and Gene Therapies: Current Status and Future Prospects. Adenoviruses.