Identity of Nucleic Acid
Nucleic acids are intricate macromolecules composed of nucleotide monomers, forming elongated chains. Therapeutics and vaccines based on nucleic acids encompass a variety of forms including mRNA, siRNA, and antisense oligonucleotides (ASOs). These modalities exhibit numerous exceptional therapeutic attributes such as remarkable specificity, functional versatility, and straightforward adaptability. Characterization and analysis of nucleic acids are pivotal in drug development, regulatory approval, and quality assurance, involving the identification of nucleic acid type, sequence, and any modifications or variations present. Creative Biolabs employs diverse approaches, including molecular weight determination and sequencing, to ensure the accuracy of oligonucleotide identity. Molecular weight and sequencing serve as essential verification methods.
Tab.1 Several approaches for identifying nucleic acids.
| Test Methods | Description | Application |
|---|---|---|
| Mass Spectrometry (MS) | MS involves ionizing compounds to create charged molecules or fragments, which are then separated by mass-to-charge ratio (m/z) and detected. | Qualitative analysis of oligo samples to determine the absence of target sequence. |
| Liquid Chromatography with tandem mass spectrometry (LC-MS-MS) |
MS sequencing ensures the accuracy of oligo sequence arrangement and modification by matching production information with theoretical sequence product ions. This powerful analytical approach integrates the separation abilities of liquid chromatography with the outstanding sensitivity and selectivity of triple quadrupole mass spectrometry. It remains invaluable for validating sequences and detecting modifications. |
Validate the sequence of nucleic acids. By separating and ionizing nucleic acid samples, and then analyzing the resulting ion mass-to-charge ratio (m/z) with a mass spectrometer, the base sequence of the nucleic acid can be determined. Detect and identify modification groups in nucleic acids, such as methylation, phosphorylation, etc. This is crucial for studying the function and metabolism of nucleic acids. Detect and identify hybridizations between nucleic acids, such as in RNAi (RNA interference) studies, to determine the hybridization of siRNA to mRNA. |
| Sanger Sequencing |
It is particularly valuable for detecting and determining the nucleotide sequences of both mRNA and plasmid DNA. Renowned for its high accuracy and reliability, it is ideal for sequencing single genes or small DNA segments. |
Identify the precise sequence of nucleotides in mRNA molecules. Confirm the integrity of plasmid constructs, verify the presence of desired DNA inserts, and ensure accurate genetic engineering. |
| Next-Generation Sequencing (NGS) | It is a high-throughput technology used for nucleic acid sequencing. It rapidly and accurately determines the sequences of DNA or RNA, generating large amounts of data at a relatively low cost. | Detect both single-stranded and double-stranded DNA as well as RNA sequences. |
| Third generation sequencing | Characterized by their capability to directly sequence single DNA molecules in real-time, without relying on PCR amplification or DNA fragment cloning, technologies such as Single-Molecule Real-Time (SMRT) and Oxford Nanopore sequencing are prominent examples. |
Detect both single-stranded and double-stranded DNA, RNA sequences, or sequence libraries within target samples. It is particularly effective for long sequences or those containing repetitive elements. Additionally, it enables the sequencing of long single-stranded DNA molecules. |
SERVICES
Creative Biolabs provides top-tier nucleic acid identity services worldwide. Our services encompass determining nucleic acid sequences, identifying mutations or modifications, analyzing subtypes or splice variants, and elucidating RNA or DNA structure and interactions. For further details, reach out to us for a quotation. We guarantee a response within 24 hours and will tailor an optimal method for your project.
