GPR150 Membrane Protein Introduction

Introduction of GPR15

GPR150 belongs to the G-protein-coupled receptor (GPCR) family which is cylindrical receptor protein usually found in the cellular membrane and is involved in signal transduction, by which chemical messengers found outside of the cell can alter intra-cellular protein activity and gene expression. The main structural characteristic of GPR150 is seven transmembrane α-helices (TMs). GPR150 is encoded by the GPR150 gene which is located at 5q15. Besides, GPR150 is comprised of 434 amino acids in human, and the mass of GPR150 is 46,353 Da.

Basic Information of GPR150
Protein Name	Probable G-protein coupled receptor 150
Gene Name	GPR150
Aliases	NA
Organism	Homo sapiens (Human)
UniProt ID	Q8NGU9
Transmembrane Times	7
Length (aa)	434
Sequence	MEDLFSPSILPPAPNISVPILLGWGLNLTLGQGAPASGPPSRRVRLVFLGVILVVAVAGNTTVLCRLCGGGGPWAGPKRRKMDFLLVQLALADLYACGGTALSQLAWELLGEPRAATGDLACRFLQLLQASGRGASAHLVVLIALERRRAVRLPHGRPLPARALAALGWLLALLLALPPAFVVRGDSPSPLPPPPPPTSLQPGAPPAARAWPGERRCHGIFAPLPRWHLQVYAFYEAVAGFVAPVTVLGVACGHLLSVWWRHRPQAPAAAAPWSASPGRAPAPSALPRAKVQSLKMSLLLALLFVGCELPYFAARLAAAWSSGPAGDWEGEGLSAALRVVAMANSALNPFVYLFFQAGDCRLRRQLRKRLGSLCCAPQGGAEDEEGPRGHQALYRQRWPHPHYHHARREPLDEGGLRPPPPRPRPLPCSCESAF

Function of GPR150 Membrane Protein

The human GPR150 was found in a sequence submitted to the nr database already in 2001 but not described as a GPCRs. In fact, GPR150 is one member of G-protein coupled receptor 1 family, and it is found to be related to the β-group of Rhodopsin family. The main bioactivity of GPR150 is mediated by G protein. Besides, GPR150 is found in mammals only, which means the receptor arise lately. The closest relatives of the GPR150 are the oxytocin and arginine-vasopressin receptors (OXTR/AVPR) which are involved in water balance, it is possible that the function of the GPR150 is essential only for land-living species. What’s more, methylated promoter CpG islands (CGIs) can be used to find novel tumor-suppressor genes and disease markers. It has been proved that, in primary ovarian cancers, CGIs of GPR150 (in 4 of 15 cancers) were methylated, so aberrant methylation of GPR150 could be candidate tumor markers.

Fig.1 The G-protein activation/deactivation cycle (Thomsen, 2005).

Application of GPR150 Membrane Protein in Literature

Furlong M. and Seong J.Y. Evolutionary and Comparative Genomics to Drive Rational Drug Design, with Particular Focus on Neuropeptide Seven-Transmembrane Receptors. Biomolecules & Therapeutics. 2017. 25(1), 57-68. PubMed ID: 28035082

This article discusses the appeal of neuropeptide-GPCRs systems as drug targets and provides an overview of concepts in the evolution of vertebrate genomes and gene families. And GPR150 is one of the drug targets.
Martin A.L., et al. Constitutive Activity among Orphan Class-A G Protein Coupled Receptors. PLoS ONE. 2015. 10(9), e0138463. PubMed ID: 26384023

This study reveals the extent of constitutive activity among orphan class A G-protein coupled receptors within the cAMP signaling pathway. The activity of GPR150 is inhibited under both baseline and forskolin stimulated.
Cai L.Y., et al. Identification of PRTFDC1 silencing and aberrant promoter methylation of GPR150, ITGA8 and HOXD11 in ovarian cancers. Life Sciences. 2007, 80(16), 1458-1465. PubMed ID: 17303177

This article reveals that CGIs of GPR150 (in 4 of 15 cancers), ITGA8 (2/15), PRTFDC1 (1/15), and HOXD11 (1/15) are methylated in primary ovarian cancers. Silencing of PRTFDC1 is revealed here for the first time, and aberrant methylation of GPR150, ITGA8 and HOXD11 could be candidate tumor markers.
Gloriam D.E., et al. Nine new human Rhodopsin family G-protein coupled receptors: identification, sequence characterisation and evolutionary relationship. BBA - General Subjects. 2005, 1722(3), 235-246. PubMed ID: 15777626

This article reports nine new members of the Rhodopsin family of human G protein-coupled receptors (GPCRs) including GPR150 found by searches in the genome databases.

GPR150 Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-GPR150 antibody development services.

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Reference

Thomsen, W. and Frazer, J. D. (2005). Functional assays for screening gpcr targets. Current Opinion in Biotechnology. 16(6), 655-665.

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