Human Transporter BCRP Fluorescence-based Antagonist Assay Service

Unveiling BCRP Interactions for Advanced Drug Development

Creative Biolabs integrates the human transporter breast cancer resistance protein (BCRP) fluorescence-based antagonist assay service into our comprehensive In Vitro ADME Service. Our ADME service includes:

The human transporter BCRP fluorescence-based antagonist assay service focuses on evaluating interactions between drug candidates and the BCRP. BCRP plays a critical role in drug disposition, influencing efficacy and pharmacokinetics across various therapeutic areas. At Creative Biolabs, we specialize in delivering precise and insightful assays for assessing BCRP function. Our dedicated service ensures thorough evaluation essential for optimizing drug development strategies.

We offer a comprehensive range of services, including:

• Functional assessment of BCRP using CHO-K1 cell-based assays
• Utilization of Hoechst 33342 as a specific fluorescent substrate for measuring transporter activity
• Application of fluorimetry to quantitatively assess fluorescence responses

Assay Information:

Workflow

• Cell Culture Preparation
  • CHO-K1 cells expressing BCRP are cultured under controlled conditions to ensure optimal expression levels of the transporter.
• Assay Setup
  • Cells are seeded into assay plates and allowed to adhere.
  • Test compounds, including potential BCRP antagonists, are prepared in appropriate concentrations for testing.
• Treatment and Incubation
  • Cells are treated with the test compounds.
  • Hoechst 33342, a fluorescent substrate actively effluxed by BCRP, is added to the assay wells.
• Incubation Period
  • The assay plate is incubated under specified conditions to allow interactions between the compounds and BCRP.
• Fluorescence Measurement
  • After the incubation period, fluorescence intensity is measured using a fluorimeter.
  • Hoechst 33342 fluorescence inside the cells indicates BCRP inhibition or modulation by the test compounds.
• Data Collection and Analysis
  • Fluorescence data is collected and analyzed to quantify BCRP inhibition or modulation by the test compounds.
  • Data analysis includes comparing fluorescence intensities between test and control samples to determine BCRP activity.

Applications

• Screening drug candidates for BCRP-mediated interactions
• Predicting drug-drug interactions and pharmacokinetic outcomes
• Optimizing therapeutic strategies to overcome BCRP-related drug resistance

Advantages

• High Sensitivity: Detects subtle changes in BCRP activity crucial for drug efficacy.
• Robust Analysis: Provides reliable data supporting informed decision-making in drug development.
• Customized Solutions: Tailored assays to meet specific project requirements and objectives.

For precise evaluation of BCRP interactions crucial to advancing drug development, partner with Creative Biolabs. Contact us today to explore how our specialized BCRP fluorescence-based antagonist assay service can accelerate your research and development efforts.

This enriched introduction provides a comprehensive background on the importance of BCRP in drug development, emphasizing Creative Biolabs' specialized assay service within a broader context of In Vitro ADME Screening. It highlights the significance of BCRP evaluation and the capabilities of our service in supporting effective drug development strategies.

For Research Use Only | Not For Clinical Use