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Magic™ Large-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for Protein Production (CAT#: MPCFK-3-7)

Datasheet
Magic™ Large-scale Cell-free Protein Expression in Wheat Germ lysate for Protein Production Kit is a cell-free protein synthesis system using wheat germ extracts for protein production. It contains 1 ml optimized and high-performance wheat germ extract for different reaction scales. This kit is premixed and allow to setup the transcription and translation reaction by just a few pipetting steps. It's a bilayer format for performing large-scale protein expression reactions.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • Different reaction scales
  • Reaction Volume
  • Different reaction scales
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts
    40xReaction buffer component 1
    40xReaction buffer component 2
    40xReaction buffer component 3
    40xReaction buffer component 4
    Creatine Kinase
    5×Transcription Reagent
    NTPmix
    RNase Inhibitor
    SP6 RNA Polymerase
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

Simple operation

Large-scale protein expression reactions are also convenient to use.
2023.1.14

Q&As

What is the reaction format of the dialysis protein expression reaction format of the wheat germ cell-free expression system?

Protein translation reactions are setup like for a batch reaction and then placed in a dialysis cup as shown in the figure or larger dialysis cassettes (not shown). Afterwards the dialysis cup or cassette is put into contact with a feeding buffer. Through the dialysis membrane, the translation reaction is continuously supplied by fresh substrates from the feeding buffer, while small molecular weight inhibitors are removed, similar to the repeat-batch method.
2023.01.26

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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