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Magic™ Large-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Labeling Reaction (CAT#: MPCFK-3-12)

Datasheet
Magic™ Large-scale Cell-free Protein Expression in Wheat Germ lysate for His-tagged Protein Labeling Reaction Kit is a cell-free protein synthesis system using wheat germ extracts. All amino acids from the wheat germ extract were removed essentially. Reaction buffer is amino acid free thus the user can control amino acids in their expression experiment. The user must purchase amino acids separately to conduct protein expression experiments. The lysate removed wheat proteins that would otherwise bind to Ni resins during the purification step.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • Different reaction scales
  • Reaction Volume
  • Different reaction scales
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts removed Amino Acids
    40xReaction buffer component 1
    40xReaction buffer component 2
    40xReaction buffer component 3
    40xReaction buffer component 4
    Creatine Kinase
    5×Transcription Reagent
    NTPmix
    RNase Inhibitor
    SP6 RNA Polymerase
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The immunogenicity of His is low

The purified protein could be used to immunize mice directly.
2023.1.19

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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