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Magic™ Small-scale Cell-free Membrane Protein Expression in Wheat Germ lysate for His-tagged Protein Production (CAT#: MPCFK-3-6)

Datasheet
Magic™ Small-scale Cell-free Protein Expression in Wheat Germ lysate for His-tagged Protein Production Kit is a cell-free protein synthesis system using wheat germ extracts for His-tagged protein production. The lysate is optimized and high-performance wheat germ extract and removed wheat proteins that would otherwise bind to Ni resins during the purification step. This kit is premixed and allow to setup the transcription and translation reaction by just a few pipetting steps. It's a bilayer format for performing small-scale protein expression reactions. The kit can be used either with plasmid DNA or with PCR DNA for protein expression.

Product Specifications

  • Lysate Type
  • Wheat Germ lysate
  • Size
  • 16 reactions
  • Reaction Volume
  • 226 µL per reaction
  • Expected Yield of Positive Control
  • 20-40 µg/ml
  • Components
  • Wheat Germ Extracts
    Reaction buffer
    Transcription Reagent
    Aluminum seal
  • Storage Conditions
  • Store at -80°C.

Customer reviews and Q&As    

Customer Review

The result of purification is guaranteed

The cleavage products do not contain wheat proteins and will not bind to Ni resin to affect the purification results.
2023.1.13

Q&As

What is the reaction format of the repeat-batch protein expression reaction format of the wheat germ cell-free expression system?

Translation reactions are setup like batch reactions with all reagents added to the same reaction vial. However, the reaction vial has a membrane at the bottom (indicated by "M" in the figure), and by centrifugation the reaction mix can be pressed through the membrane to remove small molecular weight inhibitors from the translation reaction. After the concentration step, a new reaction buffer is added to supply the translation reaction with fresh substrates. The concentration step has to be frequently repeated to make this method effective. As an alternative, the concentration step can be automated by applying pressure to press the reaction mix through a filter from time to time followed by adding new reaction buffer and RNA template ("Filter-Feed Method").
2023.01.24

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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