Custom Camelid Fc-Fusion Protein Preparation as Immunogen

Creative Biolabs has been a long-standing expert in VHH development. With over ten years of extensive experience and advanced antigen preparation platforms, our scientists are confident in designing and preparing optimized camelid Fc-fusion proteins as immunogens tailored to meet your specific project needs. By partnering with Creative Biolabs, our specific immunogen preparation strategies will enhance your VHH development programs and increase the potential for research success.

Camelid Fc-Fusion Protein Preparation at Creative Biolabs

Creative Biolabs is a well-recognized expert in high-quality antigen preparation for global customers. Selecting the proper immunogen is one of the most critical steps in a successful single-domain antibody (sdAb) development project. For target proteins that are small or have low immunogenicity, these immunogens may need to be designed and prepared in the optimized formats (such as Fc-fusion) for immunization. However, human Fc or mouse Fc, often used to increase antigen size or exposure, may lead to tag immunogenicity and reduce the possibility of in vivo VHH production against targets.

With advanced platforms and powerful technologies, our scientists are confident in overcoming these challenges to meet the demands of our global customers. We have more than ten years of extensive experience in providing a unique series of camelid (e.g., llama, alpaca, and camel) Fc-fusion proteins for immunogen preparation, ensuring a promising immune response in the camelid immunization stage. Whether you have predetermined protocols or need Creative Biolabs to develop one, our professional staff will work closely with you to ensure the success of your program. We offer technical support with exceptional reliability and the fastest turnaround time.

Features of Camelid Fc-Fusion Proteins

In recent years, there has been a dramatic increase in global interest and sales of therapeutic VHHs. As a pioneer and global leader in VHH development, Creative Biolabs is professional in applying advanced strategies for camelid Fc-fusion protein preparation to satisfy various project demands. If you are interested in our services, please do not hesitate to contact us for more details. We look forward to discussing your inquiry and finding the best solution for your needs.

Published Data

Fig. 1 Various α–Cbtx-specific VHHs and VHH2-Fc antibodies reduced lethality induced by α–Cbtx in mice.^1,2

This study examines the ability of llama-derived single-domain antibodies (VHHs) and a VHH-Fc fusion antibody to neutralize α-cobratoxin (α-Cbtx) in vivo. Researchers immunized llamas, identified high-affinity VHHs, created a VHH-Fc fusion antibody, and produced it in plants. As illustrated in Fig.1, the efficacy of various α–Cbtx-specific VHHs and a VHH2-Fc antibody in neutralizing α-Cbtx-induced lethality was assessed in mice. The results showed that all of the specific VHHs and VHH2-Fc provided either prolonged survival or complete protection against the α-Cbtx challenge when co-administered. The most efficacious antibodies were VHH C2 and VHH2-Fc, which neutralized α–Cbtx at 0.75× and 0.78× MR (molar ratio) of VHH to toxin, respectively, demonstrating 100% survival and total neutralization of α-Cbtx. These newly discovered antibodies were found to be effective in counteracting the lethal effects of α-Cbtx in vivo, potentially paving the way for the development of new antidotes for snake venom.

FAQ

1. What is Camelid Fc-Fusion Protein?

Camelid Fc-fusion proteins consist of the Fc (fragment crystallizable) region of camelid antibodies combined with other protein domains, such as the variable regions of antibodies or functional proteins. Camelids, such as llamas and alpacas, produce unique single-domain antibodies (VHHs) that can be easily fused with Fc regions to improve stability and extend half-life.

2. What are the advantages of using Fc-fusion proteins in immunogen preparation?

Fc-fusion proteins combine the high specificity of the targeting domain with the benefits of the Fc region. They are designed to target specific domains with high affinity, while the Fc region enhances solubility, stability, and half-life. This leads to improved immunogenic potential, stronger immune responses, and simplified protein purification.

3. How is a Camelid Fc-Fusion Protein constructed for use as an immunogen?

Constructing a Camelid Fc-fusion protein involves several steps. Firstly, the gene encoding the VHH or other targeting domain is fused in-frame with a gene encoding the Fc region. This construct is then inserted into an appropriate expression vector, which is introduced into a suitable host cell line (like CHO or HEK293 cells) for protein expression.

4. What are the key applications of Camelid Fc-Fusion Proteins?

They are used in vaccine development as immunogens to stimulate immune responses, in therapeutic applications where longer serum half-life is beneficial, in diagnostics due to their high binding affinity and specificity, and in research to investigate protein-protein interactions or signaling pathways.

5. How to verify the functionality of a prepared Camelid Fc-Fusion Protein?

Binding assays such as enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and bio-layer interferometry (BLI) can confirm the interaction between the VHH domain and the target antigen. SDS-PAGE and Western blotting can assess the size and integrity of the fusion protein. Furthermore, functional cell-based tests can be performed to verify biological activity.

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