Immune VHH Library Construction Service

Creative Biolabs has been long devoted to the development and application of single-domain antibody (sdAb). To meet the growing demands for VHH, Creative Biolabs has established the best solutions to generate an immune VHH library using our reliable and robust platform, brilliant technical scientists, and advanced techniques. Our scientists are confident in solving each customer's specific problems and requirements.

Advantages of Immune VHH Library

Successful enrichment of rare VHH specificities or identification of antigen-specific VHHs can be straightforward from an immune VHH library made with material from immunized donors. The generation of immune VHH libraries stands out as a highly convenient option to easily generate specific binders because the immune specificity is not shuffled during the construction of the library. The high affinity of VHH binders retrieved from immune libraries is ensured based on in vivo somatic maturation. In general, the affinities of these VHHs could be in low-nM or pM levels. Furthermore, libraries constructed from a small blood sample already represent the immune VHH repertoire of lymphocytes present in the bloodstream of the immunized animals. In practical terms, this means that the size of VHH libraries can be several orders smaller to achieve a similar representation of the original immune repertoire.

Immune VHH Library at Creative Biolabs

High-affinity VHH generation, especially from immune VHH libraries, remains a highly valued tool for most biomedical applications, including disease diagnosis, bioimaging, drug screening, and targeted therapy. The potential for obtaining antigen-specific VHHs benefits from using our dedicated libraries from immunized animals that contain a high ratio of binder to non-binder clones due to the in vivo proliferation of B-cells carrying antigen-specific VHHs.

Currently, Creative Biolabs offers a custom immune VHH library construction service to guarantee the generation of unique specificity and high affinity of VHHs. The whole procedure at Creative Biolabs, from the initial immunization to the construction of the immune VHH library, is routinely completed in less than a few months. Using our immune library is the fastest and most reliable way to obtain VHHs with high affinities. With a comprehensive network of VHH discovery and development, and experts using the most up-to-date technologies, we are your ideal VHH development program partner worldwide.

Creative Biolabs is a world leader in VHH services, and our focus is to exceed your expectations while providing accurate and reliable VHH-related services. With over ten years of experience, we are happy to help resolve any questions you may have about VHH discovery and development. Our state-of-the-art VHH solutions will bring unmatched comfort to your projects. Please feel free to contact us and learn more about our immune VHH library construction service.

Published Data

Fig. 2 Expression, Purification, and ELISA Analysis of VHH Binding to Corresponding Antigens.^1,2

This article primarily discusses the construction of an alpaca immune VHH library and the selection of VHHs against Drosophila melanogaster proteins. The researchers initially immunized the alpacas with a Drosophila embryo lysate, extracted the RNA, and synthesized the cDNA. Then, they constructed an immune VHH library with a capacity of 3×10⁷ through PCR amplification and phage display technology. Specific VHHs binding to Drosophila proteins CG7544, Myc, and CyclinE were screened using phage display and ELISA techniques, with their high affinity to the corresponding antigens validated in vitro through ELISA and surface plasmon resonance (SPR) technology. This study not only demonstrates an efficient VHH screening strategy but also provides new tools for the study of Drosophila proteins, which may advance research in developmental biology and the mechanisms of neurological disorders.

FAQ

1. What is an immune VHH library?

An immune VHH library is a collection of VHH sequences produced from a camelid's immune system after immunization with a specific antigen. This library contains a varied repertoire of heavy-chain antibody fragments unique to the antigen, allowing for the selection and identification of high-affinity VHHs for research, diagnostic, or therapeutic applications.

2. Why are camelids used for VHH libraries?

Camelids (including camels, llamas, and alpacas) generate heavy-chain-only antibodies (HCAbs). The variable section of these HCAbs, known as VHH, is small, very stable, and capable of attaching to epitopes that cannot be reached by conventional antibodies. These characteristics make camelid VHHs suitable for a variety of applications in research, diagnostics, and therapies.

3. What are the advantages of using immune VHH libraries over naive libraries?

Immune VHH libraries have more high-affinity binders because they are produced from animals that have been vaccinated with a specific antigen of interest. This immunization process increases the synthesis of VHHs with better specificity and affinity for the antigen, improving the chances of identifying effective binders.

4. How is an animal immunized to create an immune VHH library?

Camelids (like camels, llamas, and alpacas) are extensively used to produce VHHs. During the development of a VHH library, the camelids receive several injections of the desired antigen combined with an adjuvant to enhance the immune response, spanning weeks to months. Activated by specific antigens, these immunized camelids generate a diverse array of VHHs against the antigens, boosting the discovery of novel VHHs for research or clinical use.

5. What is the process for constructing an immune VHH library?

Firstly, immunize the camelid with the target antigen before collecting its blood or lymphoid tissue which is used to extract RNA. Then synthesize cDNA from the extracted RNA and utilize PCR to amplify the VHH-coding regions using specific primers. After that, begin constructing the immune VHH library by cloning the amplified VHH sequences into an appropriate phage display vector.

Resources