Encoded by the fibroblast growth factor 2 (FGF2) gene, it functions via binding to fibroblast growth factor receptor (FGFR) proteins. FGF2, or basic FGF (bFGF), controls numerous fundamental biological processes such as cell proliferation and self-renewal and also functions as one of the most critical components of commercial iPSC medium. Creative Biolabs provides two kinds of FGF2 products owning superior performance to speed up your stem cell research.
Creative Biolabs offers high-bioactivity recombinant human FGF2 (hereafter, FGF2) and a more stabilized version of FGF2 called FGF2 STAB. The latter contains 9 amino acid substitutions which greatly enhance the stability reaching a 7-day half-life even longer while keeping bioactivity. FGF2 STAB is a time-saving and cost-effective masterpiece because daily FGF2 refreshing is not required in cell culture. More information about our two FGF2 products can be checked below.
| FGF2 | FGF2 STAB | |
| Cat# | STGFBQ01 | STGFBQ01S |
| Origin | Produced by laboratory-breeding plant seeds of Camelina sativa | |
| Amino Acids | 146 amino acids | 154 amino acids |
| Referred Accession Number | P09038 | |
| Protein Molecular Mass | 16.06 kDa | 17.20 kDa |
| Reactivity Species | Human. Perhaps bovine, porcine, and mouse | Human, bovine and porcine |
| Purity | ≥ 95% measured by SDS page under reduced conditions | |
| EC50 (NIH/3T3 Proliferation) | ≤ 0.5 ng/mL | ≤ 1 ng/mL |
| Formulation | Lyophilized or PBS at 1 mg/mL | |
| Storage | Stored at -20ºC or -80ºC after reconstituted in PBS | |
| Other Key Features | Sterile, Endotoxin-free, Animal Component-Free, Tag-free | |
Fig.1 Both FGF2 and FGF2 STAB show high purity and FGF2 STAB exhibits outstanding bioactivity.
Fig.2 FGF2 STAB displays long-term bioactivity without the need of daily medium refreshing.
Creative Biolabs provides fabulous FGF2 product and cleverly designed stable version to our worldwide clients, please feel free to contact us for more information.
Not only in traditional 2D culture mode but also in 3D culture pathway forming embryonic bodies, FGF2 STAB in E8-based customized media maintains human iPSC growth in typical cell morphology (Fig. 2A). Besides, FGF2 STAB supplemented in StemFit Basic medium can fit reduced weekly feeding schedules without losing cell pluripotent compared with commercial mTeSR medium (Fig. 2B). Our cost-effective FGF2 also performs well in human iPSC culture.
Fig.3 FGF2 and FGF2 STAB can maintain human iPSC growth and pluripotent.
Compared with negative control (N.C.), all human mesenchymal stem cells (MSCs) treated with corresponding FGF2 in same concentration show stronger CD73 expression (Fig. 3A). In a 7-day cell culture trial without medium refreshing, FGF2 STAB exhibits the highest proliferation promotion indicating its outstanding thermal stability, then FGF2 group and positive control (P.C.) group (Fig. 3B). In a 21-day normal cell culture assay, all FGF2 keep a fibroblast-like morphology of MSCs and high-level CD73 expression (Fig. 3C). Similar conclusion can be generated on normal human dermal fibroblast (NHDFs) following similar workflow, such as maintenance of activated ERK/MAPK pathway reflected by high phosphorylated ERK1/2 signals (Fig. 4A, 4C), and notable growth stimulation by FGF2 STAB (Fig. 4B).
Fig.4 FGF2 and FGF2 STAB show positive growth effects on human MSCs.
Fig.5 FGF2 STAB performs best on normal growth and proliferation of NHDFs.
For Research Use Only. Not For Clinical Use.
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