B Cell Immortalization Service

Based on the leading-edge platform and profound knowledge in cell immortalization, the specialized and dedicated team at Creative Biolabs provides world-class solutions for B cell immortalization and helps you obtain the desired results. Our state-of-the-art technologies and highly qualified personnel guarantee that we can deliver maximum quality and flexibility in all phases of the service lifecycle.

Overview of B Cell Immortalization

Generation of EBV-transformed B Cells

Since it was recognized that the Epstein Barr Virus (EBV) could be used in vitro to infect human B lymphocytes and produce immortalized polyclonal B cell lines in culture, this technique has been used with great effect to facilitate immunological and molecular studies. EBV belongs to the herpesvirus family and is the main agent that causes human mononucleosis. The EBV infects B lymphocytes and immortalizes and transforms the cells into long-term growing EBV-bearing lymphoblastoid cell lines (LCLs), which consistently express the viruses.

Applications of Immortalized B Cells

The EBV-based immortalization technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As a result, EBV immortalized B cell lines are extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA, expression RNA, and protein and system biology analysis. In addition, these cell lines can be used as a culture model for various studies, including functional and serological studies, molecular and genetic research, large-scale drug library screening, vaccine study, and high throughput biological studies.

B cell immortalization has important applications in stem cell research, particularly in the generation of induced pluripotent stem cells (iPSCs). B cell immortalization can provide a virtually infinite supply of cells that can be used for reprogramming into iPSCs. In addition, B cells can be easily obtained from peripheral blood or other tissues, making them a readily available source of cells for iPSC generation. 

Services at Creative Biolabs

EBV immortalization of B cell line is considered as the method of choice for generating LCLs. Immortalized cells are important models for studies in cell biology, cellular metabolism, and differentiation; and can be used for research in cancer biology, immunology, neurology, hematology, and more. With over a decade of experience and state-of-the-art platforms, Creative Biolabs provides highly custom solutions to effectively support your B cell immortalization projects. Our outstanding QC of immortalized cell lines characterized according to your requirements includes mRNA expression, protein expression, or functional assays.

Our service utilizes state-of-the-art techniques to immortalize B cells while preserving their original functionality and characteristics.

• The process starts with the isolation of B cells from the donor sample, followed by the introduction of specific genetic modifications or viral vectors to induce immortalization. This step is carefully monitored to ensure the successful transformation of the cells without compromising their viability or integrity.
• Once immortalized, the B cells can be expanded in culture and maintained in a proliferative state for an extended period of time. Our service includes quality control measures to confirm the identity and purity of the immortalized B cell population, ensuring that researchers receive reliable and reproducible results.
• In addition to standard immortalization protocols, we also offer customization options to meet the specific needs and requirements of our clients. This includes the selection of donor samples based on desired characteristics, as well as the incorporation of additional modifications or treatments to tailor the immortalized B cells to a particular research project.

Our B cell immortalization service provides researchers with a valuable tool for advancing their stem cell research.

Features of Our Services

• Immortalized cell lines from any species and any tissues with the function you need
• Characterization according to your requirements
• Projects designed and handled by experienced scientists
• Reliable lab report with timely update
• Fast turnaround time
• Affordable price with the best quality

As an experienced expert in cell immortalization, Creative Biolabs provides custom-specific research and solutions. If you have any special needs in constructing an immortalized cell line as an iPSC research method, please contact us for this special service. Let us know what you need, and we will accommodate you. We look forward to working with you in the future.

Published Data

Below are the findings presented in the article related to b cell immortalization.

Immortalized blood cells (i.e., lymphoblastoid cell lines; LCL) can be used to generate iPSCs. Stephen J. Walker et al. detailed a slightly simplified methodology specifically designed for generating and characterizing iPSCs from LCL that have been adequately characterized and stored.

They obtained LCL and generated iPSC by transfection of the Epi5 Episomal iPSC reprogramming plasmid. The resulting cell lines were characterized by PCR, RT-PCR, immunocytochemistry, and karyotyping to provide evidence of endogenous pluripotency, and were then evaluated for the trilineage potential of four representative clones. The results indicated that all four iPSC lines were initially pluripotent and showed trilineage potential.

Fig. 1 Characterization of LCL-derived iPSCs.¹

FAQs

• Q: What types of B cells can be immortalized using your service?
  A: We can immortalize various types of B cells, including those from human and mouse origins. Whether you need naïve B cells, memory B cells, or plasma cells immortalized, our service is tailored to meet your specific requirements. We also cater to specific subtypes of B cells based on your research needs, ensuring high viability and functionality of the immortalized cells.
• Q: How long does the B cell immortalization process take?
  A: The duration of the B cell immortalization process can vary depending on several factors, including the type of B cells and the specific requirements of your project. Generally, the process takes approximately 4-6 weeks from the initial cell preparation to the establishment of a stable immortalized cell line. We provide regular updates throughout the process to keep you informed of the progress.
• Q: Do you provide technical support and troubleshooting assistance?
  A: Absolutely. We offer comprehensive technical support and troubleshooting assistance to ensure the success of your experiments with the immortalized B cells. Our experienced team is available to address any questions or concerns you may have and provide guidance on the optimal use of the cells in your research. We are committed to supporting your scientific endeavors at every step.
• Q: What are the costs associated with your service?
  A: The cost of our service depends on the specific requirements of your project, including the type of B cells, customization needs, and any additional assays or analyses required. We offer competitive pricing and provide a detailed quote based on your project specifications. Our team is happy to discuss your needs and provide a cost-effective solution that meets your research objectives.
• Q: Can you provide a detailed report of the immortalization process?
  A: Yes, we provide a comprehensive report detailing the entire immortalization process. This includes information on the initial cell preparation, viral transformation, culture conditions, and quality control measures. The report also includes data on cell viability, proliferation rates, phenotypic analysis, and any functional assays performed. This detailed documentation ensures transparency and helps you understand the process and quality of the immortalized cells.

Scientific Resources

iPSC Reprogramming

Learn More

Reference

1. Walker, Stephen J., et al. "A simplified approach for derivation of induced pluripotent stem cells from Epstein-Barr virus immortalized B-lymphoblastoid cell lines." Heliyon 7.4 (2021).

For Research Use Only. Not For Clinical Use.