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Mesenchymal Stem Cells (MSCs) Services

Overview Service Features FAQs Scientific Resources Related Services

Mesenchymal stem cells (MSC) have received significant attention in recent years with regard to their suitability for use in cellular therapies due to their high growth potential and multipotential differentiation capacity. As a trusted research partner in the field of biotherapeutics, Creative Biolabs provides cutting-edge cell therapy research services covering every stage of MSC development, including isolation, expansion, characterization, and differentiation.

Therapeutic Potential of MSCs

MSCs are a rare heterogeneous subset of pluripotent stem cells that are defined by their ability to undergo self-renewal and differentiation into cell types of mesodermal origin, including osteocytes, chondrocytes, and adipocytes. MSCs have been recognized as attractive therapeutic candidates for the treatment of various diseases (e.g., autoimmune, vascular, and degenerative conditions) due to their unique features. Firstly, MSCs are capable of differentiating into non-mesenchymal lineages, including neural cells, endothelial cells, astrocytes, cardiomyocytes, etc. The multipotency of MSCs has shown promise in the fields of regenerative medicine and tissue repair. Secondly, MSCs have been found to provide therapeutic benefit through the secretion of soluble factors to induce an immunomodulatory environment. Thirdly, MSCs have the capacity to migrate toward sites of injury and tumor microenvironments, thus allowing them to serve as delivery vehicles for targeted therapy. In addition, MSCs are widely accessible and can be expanded ex vivo conveniently with different protocols.

Clinical applications of MSCs.Fig.1 Clinical applications of MSCs.1

Services at Creative Biolabs

Minimal criteria of multipotent MSCs proposed by the International Society for Cellular Therapy (ISCT) include adherence to plastic, specific surface antigen expression (CD73+, CD90+, CD105+, CD34-, CD45-, CD11b-, CD14-, CD19-, CD79a-, HLA-, DR-), as well as multipotent differential potential under the standard in vitro differentiation conditions. The publication of this criteria offers standards by which MSCs could be functionally and phenotypically defined.

Methods for MSC isolation, processing, characterization, expansion, and differentiation are critical because they could affect the MSC quality, functionality, and plasticity. Here, at Creative Biolabs, we are proud to provide comprehensive MSC isolation, expansion, characterization, and differentiation services.

MSC Isolation and Expansion Services

Creative Biolabs is capable of providing MSCs obtained from different sources and tissues, including but not limited to bone marrow, umbilical cord blood and stroma, placenta, adipose tissue, as well as multiple dental tissues. Thereafter, MSCs are expanded in different cultures. We have established the optimal MSC isolation and expansion methods, as well as the best performing medium to provide our clients with high-quality MSCs ideally suited for different cellular applications.

MSC Characterization Services

Followed by the isolation and expansion from reliable sources, MSCs are required to be identified and characterized. To characterize and assess the quality of MSCs, Creative Biolabs can offer services including immunophenotyping analysis, karyotype analysis, short tandem repeat analysis, as well as evaluation of differentiation potential and immune modulation function.

MSC Differentiation Services

The capabilities of MSC to differentiate into both mesenchymal and non-mesenchymal tissues have made them attractive candidates for biological cell-based tissue repair approaches. As a result, the monitoring and controlling of MSC differentiation are crucial. Creative Biolabs applies optimized media and supplements to efficiently drive the differentiation of MSCs to adipocytes, osteocytes, and chondrocytes.

Features of Our Services

  • Long-term viability, multipotent potential, integrity, and uniformity of your cells are ensured
  • Amenable to the research of different cellular applications and assay endpoints
  • Adequate cell populations with the desired functionality and phenotype
  • Optimal culture insurance by ongoing technical support and a range of tailored media
  • Customized services and individualized support to suit specific research requirements

With the help of our well-established technologies and years of experience, Creative Biolabs is a well-recognized partner of choice to aid your cell therapy research. By providing either individual service modules or a fully comprehensive service to fulfill your requirements. Please don't hesitate to contact us for more information.

FAQs

  • Q: How do you ensure the quality and purity of the MSCs used in your experiments?
    A: Quality and purity are paramount in our MSC services. We utilize stringent isolation protocols and standardized culture conditions to ensure high purity. MSCs are characterized using flow cytometry to confirm the expression of specific surface markers. We also perform functional assays to verify their differentiation potential into osteogenic, adipogenic, and chondrogenic lineages. Each batch undergoes rigorous quality control to meet industry standards.
  • Q: How do you handle the genetic modification of MSCs?
    A: For genetic modification of MSCs, we employ advanced techniques such as lentiviral transduction, plasmid transfection, and so on. Our process begins with a consultation to understand your specific genetic modification needs. We then design and construct the appropriate genetic tools, followed by careful transfection or transduction of the MSCs. Post-modification, we perform thorough validation to ensure the desired genetic changes are achieved and stable.
  • Q: Can you provide MSCs from different sources, such as bone marrow, adipose tissue, or umbilical cord?
    A: Yes, we can provide MSCs sourced from various tissues, including bone marrow, adipose tissue, and umbilical cord. Each source has unique properties and advantages, and we help you select the most suitable MSC type for your research. Our team ensures the MSCs are isolated and expanded under optimal conditions to maintain their viability, purity, and functional characteristics.
  • Q: What is the turnaround time for your MSC services?
    A: The turnaround time for our MSC services varies depending on the specific service and project complexity. Typically, MSC isolation and culture services take about 2-4 weeks. Genetic modification and differentiation assays may require additional time, ranging from 4-8 weeks. We provide detailed timelines during the project planning phase and strive to meet your deadlines while maintaining high-quality standards.
  • Q: What are the typical applications of your MSC services in scientific research?
    A: Our MSC services are applied in a wide range of scientific research areas. Typical applications include tissue engineering, regenerative medicine, immune modulation studies, and disease modeling. MSCs are also used in drug discovery and toxicity testing due to their unique properties. Researchers leverage our services to investigate MSC behavior, therapeutic potential, and interactions with other cell types in various disease contexts.

Scientific Resources

Reference

  1. Pountos, Ippokratis. "Mesenchymal stem cells." Adult Stem Cells: Recent Advances. SM Group (2017): 1-15. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only. Not For Clinical Use.