With years of experience and high-end technologies, Creative Biolabs has successfully developed a series of innovative and diversified cell therapy platforms to provide fast and convenient services for our customers. Genome editing services for stem cell is one of our well-developed technologies, from which you can get professional and effective cell therapy services.
Stem cells have been considered to have tremendous potential for biological and therapeutic applications, because of their self-replicating ability and differentiation into multiple types of cells. There are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and induced pluripotent stem cells (iPSCs), which are widely distributed in multicellular organisms.
Genome editing involves the insertion, deletion, modification or replacement of DNA in the genome with the help of synthetic nucleases, the “molecular scissors”. Targeting genome alteration of stem cells in disease models is a prerequisite for utilizing the full potential of stem cells. Genome edited human iPSCs can be employed to study gene function, drug and chemical screens. Many genome-editing techniques have been launched to improve the efficiency and speed of the development of stem cells for human disease models.
Genome edited human pluripotent stem cells hold great promise for gene therapy approaches. The development of efficient technologies for stem cell genome editing has attracted massive attention. There are various technologies have been developed to provide high-quality gene editing services.
CRISPR
CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are genetic elements in bacteria containing multiple, short, direct repetitions of base sequences, which are processed and cut by Cas (CRISPR associated proteins). The CRISPR/Cas system can help the host resist foreign genetic elements such as plasmids and phages, especially in the bacteriophage infection, conjugation and natural transformation by degrading foreign nucleic acids that enter the cell. Moreover, CRISPR spacers can recognize and silence exogenous genetic elements in a manner analogous to RNA interference (RNAi) in eukaryotic organisms.
Fig.1 Genome editing using CRISPR/Cas9. (Ding, 2016)
Transcription activator-like effector nucleases (TALENs) are fusion proteins and they are produced by fusing the DNA cutting domain of a nuclease (such as FokI endonuclease) to TALE domains, which are engineered DNA-binding domain and can specifically recognize a unique DNA sequence. TALENs are featured with an array of 33 or 34-amino acid repeats. TALENs can be used in genome editing by inducing double-strand breaks (DSB), which is an important repair mechanism in cells. TALENs have been used in many genome engineering applications.
Fig.2 Diagram of TALEN technology.
Recombinant DNA technology is a direct and valid method to join together two different originated DNA molecules to produce new genetic combinations, which are of great value to science, medicine, agriculture, and industry. Techniques of recombinant DNA technology and genetic modifications have been widely used in gene therapy in serious diseases.
Aided by our well-established platforms and experienced scientists, Creative Biolabs is capable to provide different strategies in stem cell therapy. Our proven and optimized systems can help you quickly get satisfactory results without repeated trials. And our genome modification in stem cell is highly specific without off-targeting. Please contact us for more information.
Reference
For Research Use Only. Not For Clinical Use.
