TRPC7 Membrane Protein Introduction

Introduction of TRPC7

Short transient receptor potential channel 7 (TRPC7), encoded by the gene TRPC7, belongs to the subfamily C of transient receptor potential cation channels. In terms of structure, TRPC7 shares some similar characteristics with other TRPC channels, such as six transmembrane spanning segments with nitrogen (N) and carbon (C) termini residing in the cytoplasm, a pore ion path between TM5 and TM6. Only low expression of TRPC7 is detected in testis, kidney, and possibly other tissues. There are 3 isoforms produced by alternative splicing of this gene been reported.

Basic Information of TRPC7
Protein Name	Short transient receptor potential channel 7
Gene Name	TRPC7
Aliases	Transient receptor protein 7
Organism	Homo sapiens (Human)
UniProt ID	Q9HCX4
Transmembrane Times	6
Length (aa)	862
Sequence	MLRNSTFKNMQRRHTTLREKGRRQAIRGPAYMFNEKGTSLTPEEERFLDSAEYGNIPVVRKMLEESKTLNFNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARVGDALLLAISKGYVRIVEAILNHPAFAQGQRLTLSPLEQELRDDDFYAYDEDGTRFSHDITPIILAAHCQEYEIVHILLLKGARIERPHDYFCKCNECTEKQRKDSFSHSRSRMNAYKGLASAAYLSLSSEDPVLTALELSNELARLANIETEFKNDYRKLSMQCKDFVVGVLDLCRDTEEVEAILNGDVNFQVWSDHHRPSLSRIKLAIKYEVKKFVAHPNCQQQLLTMWYENLSGLRQQSIAVKFLAVFGVSIGLPFLAIAYWIAPCSKLGRTLRSPFMKFVAHAVSFTIFLGLLVVNASDRFEGVKTLPNETFTDYPKQIFRVKTTQFSWTEMLIMKWVLGMIWSECKEIWEEGPREYVLHLWNLLDFGMLSIFVASFTARFMAFLKATEAQLYVDQHVQDDTLHNVSLPPEVAYFTYARDKWWPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVIFIMVFVAFMIGMFNLYSYYRGAKYNPAFTTVEESFKTLFWSIFGLSEVISVVLKYDHKFIENIGYVLYGVYNVTMVVVLLNMLIAMINNSYQEIEEDADVEWKFARAKLWLSYFDEGRTLPAPFNLVPSPKSFYYLIMRIKMCLIKLCKSKAKSCENDLEMGMLNSKFKKTRYQAGMRNSENLTANNTLSKPTRYQKIMKRLIKRYVLKAQVDRENDEVNEGELKEIKQDISSLRYELLEEKSQATGELADLIQQLSEKFGKNLNKDHLRVNKGKDI

Function of TRPC7 Membrane Protein

Homologous with TRPC3 and TRPC6, TRPC7 is also activated by diacylglycerol (DAG), and TRPC7 is shown to be involved in DAG-evoked calcium signaling in HaCaT human keratinocytes. TRPC7 can form a receptor-activated non-selective calcium permeant cation channel and play important roles in biological processes, such as calcium and magnesium ion transmembrane transport, manganese ion transport, platelet activation, regulation of cytosolic calcium ion concentration, single fertilization, etc. Moreover, TRPC7 is a necessary mediator of pathologic cardiac hypertrophy, in part through a calcineurin NFAT signaling pathway. TRPC7 expression may influence sperm migration and motility. Fig.1 shows the schematic representation of the activation and regulation of TRPC channels.

Fig.1 Activation and regulation of TRPC channels. (Yuyang, 2014)

Application of TRPC7 Membrane Protein in Literature

Zhang X., et al. Transient Receptor Potential Canonical 7 (TRPC7), a Calcium (Ca(2+)) Permeable Non-selective Cation Channel. Adv Exp Med Biol. 2016, 898:251-64. PubMed ID: 27161232

This review gives a novel view of TRPC7 mechanisms of its activation and functions in cellular physiology and pathophysiology.
Cui L.B., et al. Morphological Identification of TRPC7 in Cardiomyocytes From Normal and Renovascular Hypertensive Rats. J Cardiovasc Pharmacol. 2016, 67(2):121-8. PubMed ID: 26371949

This article for the first time shows that TRPC7 localizes in the surface and tubular sarcolemma of cardiomyocytes in healthy adult rats and its expression is predominantly reduced in hypertrophied cardiomyocytes from renovascular hypertensive rats, suggesting that TRPC7 may play an important role in normal physiological settings in the heart.
Miyagi K., et al. A pathogenic C terminus-truncated polycystin-2 mutant enhances receptor-activated Ca²⁺ entry via association with TRPC3 and TRPC7. J Biol Chem. 2009, 284(49):34400-12. PubMed ID: 19812035

This article suggests that heteromeric cation channels are comprised of the TRPC3 or TRPC7 protein and the TRPP2 mutant induce enhanced influx of receptor-activated Ca(2+) that may result in dysregulated cell growth in autosomal dominant polycystic kidney disease.
Lavender V., et al. Increasing the expression of calcium-permeable TRPC3 and TRPC7 channels enhances constitutive secretion. Biochem J. 2008, 413(3):437-46. PubMed ID: 18452405

This article proposes that a novel role of these members of human TRPC family may be to enhance secretion either by increasing fusion at the plasma membrane or by affecting transport through the Golgi stack.
Zhang H., et al. Synergistic actions of diacylglycerol and inositol 1,4,5 trisphosphate for Ca²⁺-dependent inactivation of TRPC7 channel. Acta Pharmacol Sin. 2008, 29(1):90-7. PubMed ID: 18158870

This article suggests that the influx of Ca²⁺ through the activated TRPC7 ion channel plays a crucial role in inactivating the channel where the cooperative actions of diacylglycerol and IP3 are essentially involved.

TRPC7 Preparation Options

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Reference

Yuyang Sun, et al. (2014). Physiological Function and Characterization of TRPCs in Neurons. Cells. 3(2): 455-475.

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