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Anti-B7-H4 (XP784) h(CD28-CD3ζ) CAR-γδ T Cells (XS-0722-ZP726)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Gamma-Delta (γδ) T cells are a distinct subset of T cells based on variable chains in the T cell receptor (TCR) protein complex. Although only 1%-5% of circulating T cells are γδ T cells, these cells are very abundant on the epithelial surface. γδ T cells exhibit functional characteristics of the innate and adoptive immune systems by expressing cytotoxic receptors such as NKG2D, NKp30 and/or NKp44 and Toll-like receptors (TLRs). γδ CARs work independently of the MHC, a function that distinguishes these cells from αβ T cells. Furthermore, upon activation, γδ T cells can act as professional antigen-presenting cells to activate CD8 αβ T cells by cross-presenting tumor antigens. Given these characteristics, adoptive transfer of CAR-modified γδ T cells may be a viable potential therapeutic option.

Our CAR γδ T cells are engineered by incorporating a second-generation CAR into γδ T cells using the non-viral Sleeping Beauty (SB) transposon or CAR lentiviral transduction technology. These CAR γδ T cells are expected to enhance the tumor killing and expansion capabilities of unmodified γδ T cells.

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Specifications

  • Species
  • Human
  • Host Cell Type
  • CAR-γδ T Cells
  • Mycoplasma Testing
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
  • Shipping
  • Dry ice
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
  • Warnings
  • Avoid multiple freeze/thaw cycles
  • Research Use Only
  • Our CAR cells are for research use only, not for diagnostic or therapeutic use.
  • Quality Control
  • Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification).
  • Sterility Testing
  • All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
  • Virological Safety Testing
  • A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

CAR Design

  • Target
  • B7-H4
  • Target Species
  • Human
  • scFv Clone
  • XP784
  • scFv Host Spcies
  • Human
  • CAR Construction
  • scFv-CD28-CD3ζ
  • CAR Signaling Cassetes
  • CD28
    CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2) proteins which are expressed on antigen-presenting cells (APC). CD28 modulates the primary TCR/CD3ζ signal in a different fashion than the late costimulatory elements OX40 and 4-1BB. CD28 enhances the expression of downstream regulators that impact on T-cell proliferation, death, differentiation, and effector functions. CAR+ T cells containing the CD28 endodomain showed strikingly enhanced sustained T cell activation, growth, survival. And CD28 results in a brightly expressed, stable receptor as the transmembrane domain. Including CD28 costimulatory domains in CARs led to enhanced anti-malignancy efficacy.
    CD3ζ
    CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
  • CAR Vector Name
  • pCDCAR1
  • CAR Vector length
  • ~8kb
  • CAR Vector Type
  • Lentiviral vector
  • CAR Generation
  • Second
  • Targeting Diseases
  • Multiple Myeloma

Customize Your CAR Products

Customize Your CAR Products
Cannot find the desired product? Don't worry, just try our online CAR and CAR cell customizing system, which offers full options to meet all unique needs, including but not limited to conventional or unconventional CAR constructs, as well as a variety of vectors and cells. The customization process can be completed with just a few simple clicks, please feel free to try it out.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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