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Anti-C43H80O10 TCR, Jurkat Cell Line (TCRJ-YC0208)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

The anti-C43H80O10 TCR Jurkat cell line is a stable cell line made from the anti-C43H80O10 TCR lentivirus. The recombinant Jurkat T cell was designed to predict the MOA of C43H80O10-TCR, measure the C43H80O10-TCR specificity and screen target cells existing C43H80O10. And the product can be used to treat cytomegalovirus infection.

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Specifications

  • Cell Background
  • The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.
  • Cell Type
  • T lymphocyte
  • Formulation
  • Containing ≥ 1 X 10*6 / vial frozen cells
  • Cell Purity
  • >95%
  • Cell Viability
  • >90%
  • Mycoplasma Testing
  • The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
  • Applications
  • • Screen for activators or inhibitors of CMV signaling in a cellular context
    • Characterize the biological activity of CMV and its interactions with ligands
    • Predict the MOA of the TCR design
    • Screen and validate CMV-expressing target cells
  • Storage
  • Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
  • Handling Notes
  • Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
    Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
    Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
  • Warnings
  • Avoid multiple freeze/thaw cycles
  • Research Use Only
  • Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.
  • Quality Control
  • Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
  • Tumorgenicity
  • Positive (In vitro/vivo transformation assay)
  • Oncogenicity
  • Positive (In vitro soft agarose assay and life-time studies )
  • Sterility Testing
  • Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
  • Identity Testing
  • Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
  • Virological Safety Testing
  • A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
  • Genetic Stability Testing
  • We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

TCR Design

  • Target
  • C43H80O10
  • Introduction
  • Natural killer T cells (NKT cells), unlike conventional T cells, specifically recognize self lipid– or foreign lipid–based antigens bound to the monomorphic MHC class I–like molecule CD1d. NKT cells have been linked to microbial immunity, autoimmunity, allergy and cancer and, accordingly, they represent an important immunotherapeutic target with immense clinical potential. Type I NKT cells express a semi-invariant TCR that has an invariant α-chain and a restricted TCRβ repertoire (α-chain variable region 24–α-chain joining region 18 paired with β-chain variable region 11 (Vα24-Jα18-Vβ11) in humans, and Vα14-Jα18 paired with Vβ8, Vβ7 or Vβ2 in mice). Nevertheless, despite this restricted TCR repertoire, NKT cell TCRs bind an array of different CD1d-restricted lipid-based antigens, including phospholipids9, as well as α-linked and β-linked glycolipids. The α-glycosidic linkage that defines α-galactosylceramide (α-GalCer) and other bacteria-derived NKT cell agonists, including α-glycuronosylceramides and α-galactosyldiacylglycerols, is considered to represent a 'microbial signature', as glycolipid antigens in mammals are typically β-linked anomers. The structures of NKT cell TCRs in complex with many α-linked glycolipids have been determined, including CD1d–α-GalCer and variants thereof and α-galactosyldiacylglycerol. Although the NKT cell TCR has been shown to be relatively rigid, conformational adjustments in complexes of CD1d and α-linked antigen permit optimal engagement of NKT cell TCRs. That is, a conserved docking topology is observed whereby the NKT cell TCR shows a parallel docking mode above the F′ pocket of the CD1d antigen-binding cleft, whereas the α-linked glycosyl moiety 'sits' snug and flat against CD1d, making many direct specificity-governing contacts with the NKT cell TCR. 2-O-oleoyl-3-O-palmitoyl-1-O-alpha-D-galactosyl-sn-glycerol is a 2,3-diacyl-1-alpha-D-galactosyl-sn-glycerol in which the groups at the 2- and 3-positions are oleoyl and palmitoyl respectively. It has a role as an antigen.
  • HLA
  • Mouse CD1d
  • Common Name
  • C43H80O10
  • Target Species
  • Borreliella burgdorferi
  • TCR Clone
  • Vα14Vβ8.2
  • TCR-Host Animal
  • Mouse
  • Vector Name
  • pCDTCR1
  • Vector length
  • ~ 8 kb
  • Vector Type
  • Lentiviral vector
  • Epitope
  • 2-O-oleoyl-3-O-palmitoyl-1-O-alpha-D-galactosyl-sn-glycerol
  • Format
  • Non-Modified TCR

Customer Reviews and Q&As

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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