CMV Specific CD4+ T cell line, Donor 401 (ICZP173)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

When stimulated in vitro with CMV lysate antigen, these CD4+ T cells show a specific proliferative response along with secretion of IFN-gamma, IL-5 and IL-13, but not IL-10. A majority of the T cell population are CD45RO+, CD45RA-, CD62L+, CD127+ and CD28+.

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Donor Information

Product Name
CMV Specific CD4+ T cell line, Donor 401

Cat. No.
ICZP173

Gender
Female

Donor Age
30

Donor Health
Healthy

HLA-B
HLA-B: 18,40

HLA-C
HLA-C: 03,07

HLA-DRβ1
HLA-DRβ 1: 08,12

Blood Type
ANeg

Ethnicity
Caucasian

Additional Donor Information
Healthy CMV seropositive female non-smoker. She takes Flonase for seasonal allergies. Genotype at FcgRIIIa is F/F

Additional Information

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Preparation
When stimulated in vitro with CMV lysate antigen, these CD4+ T cells show a specific proliferative response along with secretion of IFN-gamma, IL-5 and IL-13, but not IL-10. A majority of the T cell population are CD45RO+, CD45RA-, CD62L+, CD127+ and CD28+.

Application
Flow Cytometry; MAT assays

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage
vapour phase nitrogen

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Size
1-5M cells/vial

Keywords
ICZP173; CMV Specific CD4+ T cell line, Donor 401; CMV Specific CD4+ T cell line

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.