Human CD4+ Tem cells (IC-LX008)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

T-cells are functionally divided into helper T-cells, which are CD4+ CD8- ("T4"), and cytotoxic T-cells, which are CD8+ CD4- ("T8").
T cells can also be divided based on differentiation status into 4 subsets:
1. Naïve ("Tn ", containing Tscm), which are CD27+ CCR7+ CD45RO
2. Central-memory ("Tcm"), which are CD27+ CCR7+ CD45RO+
3. Effector-memory ("Tem"), which are CD27- CCR7- CD45RO+
4. Effector ("Teff"), which are CD27- CCR7- CD45RO-
Human PBMC are separated into 4 subsets using antibodies and magnetic beads. The subsets were: CD4+CD8- T cells (T4), CD8+CD4- T cells (T8), CD4+ central memory T cells (Tcm) and CD4+ effector-memory T cells (Tem). Immediately after isolation, the subsets and the PBMC were analyzed by flow
cytometry for the composition of the subsets.

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Donor Information

Product Name
Human CD4+ Tem cells

Cat. No.
IC-LX008

Additional Information

Formulation
Containing ≥ 1 X 10*6 / vial lyophilized cells

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Application
Those CD4+ Tem cells can be used for CAR transduction for functional analysis of proliferation, CAR expression, memory and cytotoxic function.

Research Use Only
Our immune cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage
Lyophilized cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Keywords
Human CD4+ Tem cells

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.