Expanded nTreg, Donor 345 (ICZP179)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

This Treg population is obtained by ex vivo expansion of nTreg (Tregs isolated directly from blood). The expanded nTregs show the classical regulatory T cell phenotype, i.e., CD4+, CD25hi, and CD127-. In addition, the majority of the cells show intracellular expression of FoxP3 and surface expression of TIGIT. These nTregs also have the capacity to suppress in vitro T cell proliferation (e.g., anti-CD3 driven or MLR).

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Donor Information

Product Name
Expanded nTreg, Donor 345

Cat. No.
ICZP179

Gender
Male

Donor Age
40

Donor Health
Healthy

HLA-B
HLA-B: 07,40

HLA-C
HLA-C: 03,07

HLA-DRβ1
HLA-DRβ 1: 04,15

Blood Type
OPos

Ethnicity
Caucasian

Additional Donor Information
Donor is 6' 1″ and weighs 250 lbs. He is FcgRIIIa F/F

Additional Information

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Preparation
This Treg population is obtained by ex vivo expansion of nTreg (Tregs isolated directly from blood). The expanded nTregs show the classical regulatory T cell phenotype, i.e., CD4+, CD25hi, and CD127-. In addition, the majority of the cells show intracellular expression of FoxP3 and surface expression of TIGIT. These nTregs also have the capacity to suppress in vitro T cell proliferation (e.g., anti-CD3 driven or MLR).

Application
Flow Cytometry; MAT assays

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage
vapour phase nitrogen

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Size
1-5M cells/vial

Keywords
ICZP179; Expanded nTreg, Donor 345; Expanded nTreg

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.