TCR Optimization Service for Next-Generation TCR-TIL

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs utilizes its vast technological knowledge and deep industry experience to deliver thorough and efficient construction of T cell receptor -tumor-infiltrating lymphocyte (TCR-TIL) to assist with a range of cancer therapies. Meanwhile, we provide clients with a series of TCR optimization strategies, such as enhancing TCR affinity, boosting immune effects, and analyzing resistance mutations. These improvements greatly enhance TCR performance while also promoting the durability and efficacy of TCR-TILs within the body.

Introduction

The necessity of TCR optimization arises from the need to enhance the specificity, affinity, and overall efficacy of T cells against targeted antigens, particularly those expressed on tumor cells. Without proper optimization, T cells may fail to recognize and eliminate malignant cells effectively, leading to suboptimal therapeutic outcomes.

Moreover, enhanced TCRs can reduce the likelihood of off-target effects and undesirable immune reactions, which improves the safety of these treatments. By refining the TCR's ability to distinguish between healthy and diseased cells, Creative Biolabs can develop treatments that minimize collateral damage to normal tissues. This precision is vital for improving patient quality of life and achieving durable responses in chronic conditions.

To support CellRapeutics™ Next-Generation TCR-TIL Development, our TCR optimization service is also playing an indispensable role as an advanced immunotherapeutic approach. As a leading CRO company, Creative Biolabs is committed to providing our clients with advanced TCR optimization services to help them achieve breakthroughs in areas such as tumor immunotherapy and autoimmune diseases.

Fig.1：TCR Enhancement and Verification. Fig.1 TCR Optimization and Validation.^1,3

Services

At Creative Biolabs, TCR optimization is essential for advancing TCR-TIL therapies, as it enhances their potential to target and destroy cancer cells while maintaining therapy safety. As research in our labs continues to evolve, ongoing efforts in TCR optimization hold the promise of more effective and personalized treatment options for various TCR-TIL construction. Our TCR optimization services encompass, but are not limited to, the following areas:

High-Throughput Screening

We utilize cutting-edge high-throughput screening technology to rapidly identify and select TCRs with high affinity and specificity, ensuring effective targeting of tumor antigens.

Computational Biology Support

By integrating bioinformatics and computational biology, Creative Biolabs leverages algorithms to predict the optimal TCR sequences, enhancing therapeutic efficacy while minimizing adverse reactions.

TCR Library Construction

Creative Biolabs has constructed a diverse TCR library that includes receptors sourced from healthy individuals as well as tumor patients, providing our clients with a variety of options to meet different research needs.

Functional Validation

Using molecular cloning and cell culture techniques, our scientists conduct functional validation of the selected TCRs to ensure they exhibit desirable anti-tumor activity both in vitro and in vivo.

Preclinical Research Support

Creative Biolabs offers comprehensive support for preclinical research, from animal models to pharmacokinetic studies, ensuring that the optimized TCR therapies can smoothly progress to preclinical stages.

Mass Cultivation and Expansion

Creative Biolabs uses advanced cell culture and expansion technologies that allow for the large-scale cultivation of T cells with TCR transduction. This ensures that we can provide a sufficient quantity of reliable quality cell therapy products for clinical applications. Our process employs the latest culture media and optimized workflow designs to maximize cell viability and efficacy.

Fig.2：Replacement of Three Framework Residues Reduces TCR Mispairing and Enhances TCR Expression. Fig.2 Substituting Three Framework Residues Decreases TCR Mispairing and Improves TCR expression.^2,3

Features

Comprehensive Service System: Our TCR optimization service covers the entire process, including target selection, TCR gene cloning, cell transduction, amplification, and functional validation, ensuring excellence at every stage.
Cutting-edge Technology Platform: Creative Biolabs is equipped with industry-leading genome editing technology, allowing for precise modifications of TCRs to enhance their therapeutic potential.
Interdisciplinary Team: Creative Biolabs boasts a multidisciplinary team of experts in immunology, molecular biology, and clinical medicine, enabling a multifaceted approach to problem analysis and resolution, thereby ensuring the scientific rigor and feasibility of research plans.
Comprehensive Monitoring and Quality Control: Throughout the entire service process, our staff strictly adhere to quality control procedures. From TCR construction and cell transduction to final cell expansion, each step has a dedicated monitoring mechanism in place.

Creative Biolabs is dedicated to providing efficient and precise TCR-TIL treatment services to various research institutions and pharmaceutical companies, supporting the development of safer and more effective treatment solutions. Our TCR optimization service encompasses the entire process, from TCR design and selection to functional validation. Whether you are a research institution, a pharmaceutical company, or exploring new opportunities in cell therapy, we invite you to contact us.

References

Baulu, E., et al. "TCR-engineered T cell therapy in solid tumors: State of the art and perspectives. Sci. Adv. 9, eadf3700." (2023).
Thomas, Sharyn, et al. "Framework engineering to produce dominant T cell receptors with enhanced antigen-specific function." Nature Communications 10.1 (2019): 4451.
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