Tetanus Toxoid Specific T Cells, Donor 213 (ICZP268)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

These CD4+ T cells are specific for a peptide (QYIKANSKFIGITEL) derived from tetanus toxoid bound to HLA-DRB1*01. They also recognize whole tetanus toxoid but only when presented by dendritic cells, not B-LCL. The T cells proliferate when stimulated with antigen and APC. They also produce IFNg, IL-5, IL-10, and IL-13.

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Donor Information

Product Name
Tetanus Toxoid Specific T Cells, Donor 213

Cat. No.
ICZP268

Gender
Female

Donor Age
27

Donor Health
Healthy

HLA-A
HLA-A: 02:01,02:01

HLA-B
HLA-B: 35,56

HLA-C
HLA-C: 01,04

HLA-DRβ1
HLA-DRβ 1: 11,01

Blood Type
ONeg

Ethnicity
Caucasian

Anticoagulant
Unknown

Additional Donor Information
Healthy female donor, CMV negative. 5' 10″ tall, 210 lbs. Her genotype at Fc gamma RIII alpha 158 is F/V.

Additional Information

Cell Purity
>95%

Cell Viability
>90%

Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Preparation
These CD4+ T cells are specific for a peptide (QYIKANSKFIGITEL) derived from tetanus toxoid bound to HLA-DRB1*01. They also recognize whole tetanus toxoid but only when presented by dendritic cells, not B-LCL. The T cells proliferate when stimulated with antigen and APC. They also produce IFNg, IL-5, IL-10, and IL-13.

Application
Flow Cytometry; MAT assays

Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.

Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.

Storage
vapour phase nitrogen

Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Warnings
Avoid multiple freeze/thaw cycles

Size
1-5M cells/vial

Keywords
ICZP268; Tetanus Toxoid Specific T Cells, Donor 213; Tetanus Toxoid Specific T Cells

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.