Irradiated PBMC, Donor1 (ICZP001)

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Irradiated peripheral blood mononuclear cells (PBMC) are normal PBMCs that have been exposed to 25 Gy radiation from cesium 137. This dose of radiation causes enough DNA damage to prevent cells from dividing, but still produces cytokines and other soluble factors. These PBMCs are well suited for use as feeder layers for antigen presenting cells or other cells because their lack of proliferation prevents them from contaminating the cells of interest. The irradiated PBMC can be used in combination with an antiCD3 antibody to stimulate activation and proliferation of T cells, or as a stimulator in a mixed lymphocyte reaction (MLR). In some cases, we have unirradiated cells from the same donor that can be used as controls.

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Donor Information

Product Name
Irradiated PBMC, Donor1

Cat. No.
ICZP001

Gender
Male

Donor Age
47

Donor Health
Healthy

HLA Type
HLAA: 02:01,24:02; HLAB: 07:02,15:01; HLAC: 03,05; HLADRβ 1: 04:01,13:01

Blood Type
APos

Ethnicity
Caucasian

Anticoagulant
Citrate

Additional Information

Additional Information
The donor smoked 2 cigarettes a day. His FcγRIII alpha genotype is F/V. He is allergic to dogs and penicillin.

Preparation
We obtained peripheral blood mononuclear cells (PBMC) from many donors. Our PBMCs were collected by leukapheresis and further treated with a Ficoll gradient and several washes to remove platelets.

Application
Flow Cytometry

Quality Control
The cells provided by our company are taken from blood products and separated according to standard operating procedures to ensure the stability of cell quality between batches.
Cell purity>95%
Cell viability>90%
Microorganisms, HIV, HBV, HCV, syphilis, etc. tests were performed.

Handling Notes
1. After receiving the cells, please check whether the cell bottle is intact, whether the frozen cells are thawed, whether the fresh cell culture solution has leakage or turbidity. If any of the above phenomena occur, please contact us in time.
2. Even when the test report is negative, when handling the cell sample, take precautions and complete the operation in the fume hood.
3. The cell can only be used for scientific research and should not be used for in vitro diagnosis of humans and animals.
4. During the cell culture process, please perform under aseptic conditions.

Size
05-10M cells/vial

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.