Complement Testing Services

Creative Biolabs has extensive experience in complement test, providing customers with a full range of test services, including complement function/activity test, autoantibody test, genetic test, and complement multiplex assay. We can bring consistent and efficient test services and results that can enhance your research.

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What is the Complement Test?

Complement testing is a method used to assess the function of the immune system. The complement system is a complex protein network system consisting of more than 30 soluble proteins, membrane-bound proteins and complement receptors. Complement tests mainly include the following methods: Complement function or activity assay - to assess the overall function of the complement system. Complement factor concentration assay - to measure the amount of specific complement proteins. Complement activation product assay - to detect fragments or shear products produced by the activation of the complement system. Complement autoantibody assay - to examine the presence of autoantibodies against complement proteins. Complement gene analysis - to study gene variants associated with complement.

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Service Portfolio

Complement Function/Activity test

At Creative Biolabs, all 3 pathways are tested simultaneously on all samples (CH50/AP50 hemolytic assays measure the function of the classical and alternative pathway, and ELISA assays measure the function of the lectin pathway). In addition, individual complement components and activation products are also tested through many methods.

Total Complement Activity Test

We provide functional complement testing services for classical, lectin, and alternative pathways. We use a variety of different technologies, such as ELISAs, hemolysis tests, and liposomal lysis, to meet the rigorous testing requirements of each pathway.

Individual Components Activity Test

PWe provide our customers with routine quantitative tests and functional activity tests for individual components as well as a full range of complement components testing services, including but not limited to C1q, C2, C3, C5, Factor B, Factor D, Factor H, Factor I, Factor P.

Complement Activation Products Test

Creative Biolabs has developed a variety of specific monoclonal antibodies that can be used for the testing of complement activation products. We provide our customers with top-quality complement activation products test services through a range of exclusive technologies.

Hemolytic Inhibition Assay

To support the complement system research, Creative Biolabs provides rapid hemolytic assay and hemolytic inhibition assays for complement function or activity assessment. For the samples that are not suitable for mailing, we also provide ready-to-use hemolytic assay products and guidance.

Complement Inhibitor Validation

Complement inhibition test or complement inhibitor validation is necessary to measure the effect of complement inhibitors. We provide highly custom solutions for complement inhibitors validation to effectively support your anti-complement therapeutics development.

Receptor Ligand Binding Assay

The complement receptor-ligand binding assays are valuable in understanding the molecular interactions between complement receptors and their ligands. With advanced platforms and standard procedures, we provide rapid and customized complement receptor-ligand binding assays.

Cell-based Complement Activity Assay

Creative Biolabs offers high-quality cell-based complement activity assays to help your research and drug development projects. Our services represent a comprehensive solution for researchers seeking to understand and manipulate the complex dynamics of the complement system.

Complement Autoantibody Test

Autoantibodies target a variety of complement components, convertases, and regulators. Most of these complement autoantibodies tests are based on ELISA, while the rest can be measured by other methods, such as immunofixation electrophoresis and hemolytic assays

Complement Genetic Test

Gene mutations play an important role in uncontrolled activation of the complement pathway. Complement genetic test methods have evolved into high throughput methods, such as NGS. Still, in some cases, Sanger sequencing remains the gold standard for complement gene analysis.

Complement Multiplex Assay

Our experienced technical team is confident in providing you with customized complement multiplex assay services, which cover a variety of complement proteins, to ensure the assay of multiple complement proteins of interest at the same time.

Why Choose Our Complement Testing Services?

Why Choose Our Complement Testing Services? With abundant experience in complement test, Creative Biolabs can bring consistent and efficient test services and results that can enhance your research.

Have a robust and standardized assay platform

Scientists experienced in developing complement assays

Expertise in various different technologies and methods

Delivering fast, reliable and objective, easy to interpret results

Advanced Protocols for Complement Test

Why Choose Our Complement Testing Services? We briefly summarize the complement-related assay protocols. Find complement-related assay protocols by clicking the following items.

By Target By Assays

C1q	C1r and C1s	C2	C3
C3a	C4	C4a	C5
C5a	C5b-9	C6	C7
C8	C9	Factor B	Factor D
Factor H	Factor I	Factor P	MCP/CD46
DAF/CD55	CD59	Clusterin	C1 Inhibitor
C4BP	CR1/CD35	CR2/CD21	CR3 (CD11b/CD18)
CR4 (CD11c/CD18)	C5aR (CD88)	C1q Receptor	Mannan-Binding Lectins
MASP-1 and MASP-2	Ficolins	Surfactant Protein A (SP-A)	Surfactant Protein D (SP-D)

Classical Pathway	Alternative Pathway	MBL Pathway	Ficolin-3-Pathway
Hemolytic Assay for C4	Hemolytic Assay for C3	Hemolytic Assay for C2	Hemolytic Assay for C1 Complex
Hemolytic Assay for Terminal Components	Propidium Iodide Assay	Optimizing Lysis Specific Assay	Calcein Release Assay
Decay-Accelerating Activity Assay	Cofactor Activity Assay

Applications of Complement Test

Complement Activity Assay

Assess complement activity in the classical pathway
Assess complement activity of the alternative pathway
Assess the complement activity of the lectin pathway

Complement Component Assay

Concentration of C3, C4, C1q and others
Concentration of anti-C1q antibody, anti-CFH antibody and others

Drug Discovery Support

Evaluate the in vitro efficacy of complement inhibitors, e.g., C3 inhibitors, CFB inhibitors, etc.
Perform preclinical and clinical stage pharmacodynamic analyses

Mechanistic Studies

Analyze the role of different complement activation pathways in disease
Study the interactions between the complement system and other immune responses

Disease Modeling Studies

Evaluate changes in the complement system in models of autoimmune diseases, inflammatory diseases, and other diseases
Study the role of the complement system in tumor immunity

Case Studies of Complement Test

Case 1

Functional evaluation of complement activation through hemolytic assay techniques

To investigate complement activation initiated by antibodies under therapeutic hypothermia conditions, researchers performed functional hemolytic assays at these temperatures. Specifically, they tested antibody-sensitized sheep erythrocytes incubated in normal human serum and assessed hemolysis. In this CH50 assay, the degree of hemolysis varied notably with temperature changes.

Workflow of hemolytic assay for functional analysis of the CP. (Creative Biolabs Original)

Fig. 1 Functional hemolytic assays of complement activation.¹

Fig. 2 A representative Western blot analysis of membrane-bound C3-fragments.¹

Fig. 3 C1s activation by heat-aggregated IgG for increasing temperatures.¹

Reference

Shah, Tushar A., et al. "Clinical hypothermia temperatures increase complement activation and cell destruction via the classical pathway." Journal of translational medicine 12 (2014): 1-10. Distributed under Open Access license CC BY 2.0, without modification. https://doi.org/10.1186/1479-5876-12-181 .

What Our Clients Say

“Creative Biolabs’ complement assay service is very professional. Their CH50 and AP50 test results are accurate and reliable, providing us with important support for our research. The technical team is very responsive and was able to answer our questions in a timely manner.”

— Dr. Sarah L., Immunology Researcher

“We have been using Creative Biolabs' complement concentration measurement services. Their C3 and C4 quantitative results are stable with low lot-to-lot variation, which is very important for our drug development.”

— Dr. Emily C., Clinical Researcher

“Creative Biolabs' complement assay service has helped us establish our own complement function assessment system. They not only provide testing, but also train our technicians, which is a very comprehensive service.”

— Mark T., Senior Scientist, Biotech Firm

“We used their services in the development of our complement-targeting drugs, and the sensitivity of Creative Biolabs' complement activity assay helped us to quickly screen validated candidate molecules. Their professional advice helped us throughout the process.”

— John D., R&D Director, Pharmaceutical Company

“Creative Biolabs' complement assay service has played an important role in our research on autoimmune disease mechanisms. They were able to customize the assay according to our needs, which made the service very flexible. The data analysis reports are also very detailed, which makes it easier for us to conduct follow-up studies.”

— Dr. James H., Immunotherapy Developer

FAQ about Complement Test Services

What experimental methods can be applied to complement analysis in an experimental setting?

(a) In vitro experiments with human serum and blood. We can easily study the activation mechanism and intervention of complement activation in vitro with human serum. (b) Animal experiments. We can apply genetically defective or knockout animals, such as those with genetic defects in individual complement components. (c) Testing animal complement. Hemolysis assays can be designed for any species.

What types of samples can I submit for testing?

We can work with a variety of samples, including serum, plasma, whole blood, and tissue samples. Please prepare them according to our guidelines to ensure the best results.

What are the special requirements for sample collection and processing?

Complement assays require high quality samples. Blood samples should be separated from serum within 2 hours of collection and frozen quickly. Avoid repeated freezing and thawing to prevent decreased complement activity. Fresh serum may be required for some tests.

Can multiple complement components be tested at the same time?

Our custom complement multiplex assay services allow you to simultaneously assay two or more complement proteins, including complement C2, complement C4b, complement C5a, complement C5, complement C9, complement mannose-binding lectin (MBL), complement factor D, complement factor I, and complement factor P.