Rat Complement C4b ELISA Kit-CTK-257(Cat#: CTK-257)

Name: Rat Complement C4b ELISA Kit-CTK-257
SKU: CTK-257

This product is an ELISA kit for the determination of Rat Complement C4b in the samples of Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate. It is highly sensitive and available for the detection of Rat Complement C4b as low as 25.6 pg/mL.

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Specifications

Assay Type	Sandwich ELISA
Sensitivity	25.6 pg/mL (62.5-4000 pg/mL)
Pack Size	96 tests
Species Reactivity	Rat
Target	C4b
Cross Reactivity	No significant cross-reactions have been observed between Rat Complement C4b and analogues, but existing techniques may limit the detection of cross-reactions with untested analogues.
Applications	ELISA
Storage	The storage of components varies. Please refer to the instructions for details.

Information

Introduction

Complement C4B is a non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

Customer Review

The kit has exceeded our expectations

Excellent

The reagents are stable, and the assay is very sensitive. We were able to detect subtle changes in C4b levels in our experimental samples, which was critical for our study. The customer support was also excellent, providing prompt and helpful responses to our queries.

An invaluable tool for our lab's research on complement activation

Excellent

As a researcher working on immune mechanisms, I found the ELISA kit to be extremely useful. The kit is user-friendly, with a well-documented protocol that makes the setup and execution of the assay straightforward. The results are reliable and reproducible, which is essential for publishing high-quality research.

The sensitivity and specificity of the assay are impressive

Excellent

It can detect low levels of C4b in our samples. The kit is well-packaged, and the reagents are of high quality. The clear instructions make the assay easy to perform, even for those new to ELISA.

The technical support team has also been very responsive and helpful

Excellent

I have had a great experience with the kit. The assay is straightforward to perform, and the results are consistent and reproducible. The sensitivity of the kit allows us to detect low levels of C4b, which is crucial for our research. The customer support has also been very helpful and responsive.

For Research Use Only. Not for Diagnostic or Therapeutic Applications.