This product is a 41.6 kDa recombinant mouse Complement C3c protein with His tag(s) expressed in the E. Coli.
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Expressed Host | E. Coli |
Specie Reactivity | Mouse |
Target | C3c |
Molecule Mass | 41.6 kDa |
Purity | >95% as determined by SDS-PAGE |
Formulation | PBS, pH 7.4, 1 mM DTT, 5% Trehalose, 0.01% Sarcosyl, ProClin 300 |
Product Form | Lyophilized |
Storage | Store at 2 to 8°C after reconstitution for short term (1-2 weeks). For long term storage, aliquot and store at -20 or -80°C. Avoid repeated freezing and thawing cycles. |
Introduction | Complement C3c is a cleaved product of complement C3. During the activation of complement system, C3 is cleaved into C3a and C3b fragments by C3 convertase. The C3b fragment can be degraded into iC3b when cleaved by complement factor I. iC3b serves as 'inactive' C3b unable to associate with complement factor B, which prevents amplification of the complement cascade through the alternative pathway. iC3b will be cleaved by trypsin-like enzymes releasing C3c and C3dg, and C3dg can be further cleaved into C3g and C3d. |
Alternative Names | Complement C3c; C3c fragment |
Gene ID | 12266 |
UniProt ID | P01027 |
It's a reliable and high-quality product that we will continue to use in our researchExcellentThis recombinant protein has significantly improved the efficiency of our complement pathway studies. The His tag facilitates easy purification and ensures high purity, which is essential for our in vitro assays. We've noticed a consistent performance in all our experiments, and the protein has proven to be very stable.
This product has completely changed my perspectiveExcellentThe C3c protein-His tag is of remarkable quality, with excellent purity and activity. The His tag simplifies the purification process, and the protein performs consistently in our functional assays. This has become a staple in our lab for complement system studies.
A reliable product that delivered consistent resultsExcellentThe product arrived promptly and was well-packaged. The protein showed excellent stability and maintained its activity throughout my assays. I appreciated the detailed datasheet provided, which helped in optimizing my experimental conditions.
I will definitely reorder this protein for future experimentsExcellentThis recombinant C3c protein exceeded my expectations in terms of purity and functionality. I used it for in vitro complement activation assays, and the results were highly reproducible. The His tag facilitated easy purification and detection, saving me a significant amount of time.
I am very satisfied with this product and the technical support provided by the supplierExcellentThe protein was highly pure and active, which was evident from the sharp bands in my SDS-PAGE and the strong signals in my functional assays. The His tag allowed for easy handling and reduced preparation time. This product is a valuable addition to my research toolkit.