Adenovirus-Based cDNA Expression Libraries Generation Service
Creative Biolabs always provides high-quality products and services to life science researchers all over the world with a rigorous research attitude. We have an experienced adenovirus research team that can provide you with high-standards, high-quality adenovirus-based cDNA library construction services to meet your experimental requirements.
Introduction of cDNA Expression Libraries
All mRNA transcribed at a certain stage of development is catalyzed by reverse transcriptase to obtain all cDNA fragments of the expressed protein of the organism at this stage, and these fragments are ligated into a vector and then expressed in bacteria or viruses, then the resulting set is called a cDNA library. Each bacterial or viral clone containing recombinant DNA contains only one mRNA information, and the sum of a sufficient number of clones contains the entire mRNA information of the cells. The cDNA library facilitates cloning and bulk amplification, from which the desired gene expression of interest can be screened. The cDNA library is convenient to study the expression status of the genome in the specific type of cells and the functional identification of the expressed genes. Such advantages have made it widely used in the study of important life activities such as individual development, cell differentiation, and cell cycle regulation.
Adenovirus-Based cDNA Expression Libraries
Since its use as a gene vector in the 1980s, adenoviral vectors have been widely used as gene transfer tools in vaccination, gene therapy, and functional studies. Adenoviral vectors are ideal for gene transfer primarily because they are easy to produce at high titers. It has high infectivity and effective expression of foreign genes in various cell types in vitro and in vivo. In addition, with the development of various technical means, such as the increase in the size of insert, the extension of expression time, further expand their application range, making them a powerful molecular biology tool.
Despite their obvious advantages, the construction of adenoviral vectors remains a long and time-consuming process, making it difficult to produce large numbers of clones. The homologous recombination process usually in the cell undergoes a large number of plaque purification processes and is easily contaminated by the replication-complementary adenovirus. There are currently two highly feasible methods: one is an adenoviral vector cDNA library based on the recombination between the Cre-lox shuttle plasmid cDNA library and the adenoviral genome; the other is based on the SMART library construction method, the cDNA was ligated to the vector and transformed into E. coli competent cells to obtain a high capacity library.
Creative Biolabs' adenovirus-based cDNA expression libraries generation service will provide you with a fast and efficient way to get the ideal recombinant adenoviral vector. It lays a good foundation for your subsequently functional gene screening, gene cloning, and molecular chip preparation experiments. Our services involve mRNA purification, RT-PCR, transformation and library evaluation. If you have any questions or have any difficulties, you can contact us by email or send us an inquiry to find a complete solution.
