Adenovirus Vector Virus Particles Determination
Adenovirus vectors virus particles determination actually is the quantitation of the total physical adenoviral particles. With first-in-class technologies and experienced scientists, Creative Biolabs has successfully developed a versatile gene therapy platform. Based on these cutting-edge platforms, we can provide our worldwide clients with the largest and most diverse portfolio of standard or customized adenovirus vector services based on the values of quality, timing, and price.
Adenovirus Vectors Virus Particles Count
Adenovirus vectors have made gratifying achievements in gene therapy since the 1960s. Construction by deleting certain specific gene sequences, recombinant adenovirus is produced to carry or transfer foreign genes into hosts' cells. There several features that make adenovirus an ideal therapeutic gene vector, such as low pathogenicity in humans, entry into cells via receptor-mediated endocytosis, non-integration into the host cell genome, large accommodation for inserted DNA segments, etc. Owing to these outstanding advantages, numerous efforts have been made to continuously improve the construction of adenovirus vector to achieve better therapeutics for cancer and other genetic diseases.
Adenovirus vectors virus particles generally refer to the total number of adenovirus, comprising not only live adenovirus particles or infectious adenovirus but also dead adenovirus particles. This is distinct to that adenovirus vector titer which only indicates and measures the infectious virus particles. Adenovirus vectors virus particles count is also an important parameter for characterizing adenoviral vectors construction.
Figure 1. Scheme for the generation of the E1-substituted adenoviral vectors. (Zhang, 1999)
Adenovirus Vector Virus Particles (VP) Determination Services
There are a variety of approaches for the quantitation of infectious adenovirus vectors, such as plaque assays, immunofluorescence foci assay, serial dilution method, and so forth. Increasing investigations indicated that adenovirus might form hollow or non-infectious viral vectors due to genomic deletion or mutation during packaging. The proportion of these non-infectious adenoviruses in the total number of viruses or the ratio of live/dead adenovirus is of great biological importance, which means the efficiency of virus construction and has an influence on the results of in vivo and in vitro research.
Creative Biolabs can provide several methods for rapid adenovirus vector virus particles determination, some of which are listed as follows:
- The most commonly used ultraviolet absorption (260 nm) method: determining the total adenovirus particles by calculating the optical density (OD) or the absorptivity/extinction coefficient.
- Direct determination of the physical viral particles by flow cytometry or transmission electron microscopy.
- Indirect determination of the physical adenovirus particle count by using fluorescent dye-based microplate assay rapid quantitation of adenovirus DNA (Murakami, 1999).
Services
Adenovirus vector is another extensively used gene therapy tools second only to lentivirus vector. As your best partner, Creative Biolabs is very glad to revolutionize your gene therapy project and promote your success based on our abundant experience and expertise. Our professional staff is always ready to provide you with diverse customized adenovirus vector-based gene therapy services.
If you are interested, please directly contact us or send us an inquiry.
References
- Zhang, W.W. (1999). Development and application of adenoviral vectors for gene therapy of cancer. Cancer Gene Therapy. 6(2): 113-138.
- Murakami, P.; McCaman, M.T. (1999). Quantitation of Adenovirus DNA and Virus Particles with the PicoGreen Fluorescent Dye. Analytical Biochemistry. 274: 283-288.
