Custom Bridged Nucleic Acid Synthesis

As a pioneer in oligonucleotide synthesis, Creative Biolabs has been working to develop new oligonucleotide-based technology that can manufacture compounds with superior binding affinity and chemical/biological stability. With our proprietary nucleic acid technology, we provide customized third-generation nucleic acid analogs, bridged nucleic acid (BNA) synthesis services to researchers worldwide to meet their specific needs.

What Is BNA?

There has been a growing interest in developing chemically modified nucleotides for diagnostics or therapeutics. Among the list of artificial nucleotides, BNA appears to be the most promising nucleic acid analog to date in view of the usage for applications. BNAs are a recently developed class of artificial nucleic acid analogs with high affinity to ssRNA, DNA, dsDNA. They contain a five-membered or six-membered bridged structure with a "fixed" C3'-endo sugar puckering. This bridge binds to the 2', 4'-position of the ribose to obtain a 2', 4'-BNA monomer. The monomers can be incorporated into oligonucleotide polymeric structures by standard phosphoramidite chemistry. The incorporation of BNAs into oligonucleotides allows the production of modified synthetic oligonucleotides with:

1. Higher binding affinity against their complementary strands with excellent single-mismatch discriminating power;
2. Stronger and more sequence selective triplex-forming characters, and higher nuclease resistance, even higher than Sp-phosphorothioate analogs;
3. Good aqueous solubility when compared to conventional DNA or RNA oligonucleotides.

BNA can be used to replace the first generation of BNA called Locked Nucleic Acids (LNA). So far, the third generation of BNA (2', 4'-BNA-NC) has been developed. 2', 4'-BNA-NC can be easily incorporated into oligonucleotide strands, which allows for designing BNA hybrid oligos to satisfy the need for very high and sequence-specific hybridization with nucleic acids. In addition, it exhibits superior binding affinity for ssRNA, a better binding affinity for dsDNA and a strong nuclease-resistant property. BNA's high affinity for nucleic acids and nuclease resistance property makes BNA a superior tool for developing high-value detection systems and therapeutic products.

Custom BNA Synthesis

BNA oligonucleotides offer greater flexibility when designing primers and probes. They can be mixed with DNA, RNA and other nucleic acid analogs using standard phosphoramidite synthesis chemistry. With over 10 years of experience in producing custom oligonucleotides for research, preclinical and clinical use, Creative Biolabs provides one-stop customized BNA synthesis services from primer design and synthesis to final result delivery.

For all customized BNA, we offer different scales and modifications:

• Scale
  • We provide small-scale and large-scale RNA synthesis services upon request.
• Modification/Labeling
  • General modifications, such as modified bases, phosphorylation, thiolation, linkages, etc.
  • Labeling, such as DIG, fluorescent dyes, biotin, amino-linkers, etc.
• Purification
  • Our RNase-Free HPLC purification is carefully performed in an RNase-free environment. Reagents, equipment and processing are all constantly monitored to avoid RNase contamination.
• Synthesis quality
  • All custom BNAs are manufactured under strict SOP quality control processes and checked for their quality.

With our unparalleled expertise and experience in BNA oligodeoxynucleotides synthesis, Creative Biolabs should be your first choice for customized BNA synthesis. To learn more about our service, please contact us.