Custom In Vitro Study Service for Synthetic Circular RNAs
Creative Biolabs is dedicated to expanding the study of circular RNAs (circRNAs) across a range of applications. Therefore, with our well-established platforms, we are confident in providing in vitro study services for synthetic circRNAs to global customers to facilitate your project progress.
Functions of CircRNAs
These molecules are a special class of RNAs that are stable and ubiquitous in eukaryotic cells. Most of them are noncoding RNAs (ncRNAs). According to the composition, circRNAs are divided into exons (ecircRNAs), exons-introns (EIcircRNAs) or introns (ciRNAs). The primary function of circRNA is to serve as a microRNA sponge. In addition, circRNAs are also key regulators of translation as well as protein activity. Importantly, circRNAs may also be linked to diverse diseases, including cancer and cardiovascular diseases. Synthetic circRNAs have gradually emerged as promising tools for studying circRNAs. Such circRNAs can encode proteins, which provides broad prospects for circRNAs as gene expression platforms.
Fig.1 The functions of circRNAs.1
Significance of circRNA In Vitro Study
Based on the research on the structure and function of circRNAs, some scientists have found that they play vital roles in the occurrence of diseases such as atherosclerosis, nervous system disorders, diabetes and tumors.
- The unique competitive endogenous characteristics of circRNA can provide new ideas for drug discovery.
- The tissue specificity and stability of circRNA may make circRNA an attractive biomarker.
- CircRNA research provides a new perspective on the evolution of life.
What Can We Do?
Currently, different in vitro methods for overexpressing or knocking down circRNAs are available at Creative Biolabs. Overexpression can be performed by the delivery of plasmids containing exons that produce circRNAs in animals or cells. To efficiently and safely deliver circRNAs to target organs, we employ various technologies including adeno-associated virus (AAV), nanoparticles and adenoviruses, thereby providing validation studies for the delivery of synthetic circRNAs. At Creative Biolabs, small interfering RNAs (siRNAs) and short hairpin RNAs (shRNAs) have been widely used in circRNA functional studies to trigger RNA interference on various circRNAs in vitro.
Fig.2 The role of circRNAs is correlated with hallmarks of cancer.²
Key Advantages
- We provide a variety of circRNA products. Custom exclusive circRNAs according to specific requirements is achievable.
- Design and develop in vitro experiments for synthetic circRNAs to verify their functions.
- Various cell models including induced pluripotent stem cell (iPSC)-derived cell models, primary cells, cancer cell lines and immune cells are available.
- Customized reasonable solutions according to different research, such as tumor killing and cardiovascular disease research.
Creative Biolabs is an innovative company dedicated to the research of gene therapy. We focus on the development of circRNAs, contributing professional knowledge and advanced analysis technologies for comprehensive solutions. Our vision is to provide trustable solutions and custom in vitro study services for synthetic circRNAs to help global customers make major breakthroughs in the field of RNA research. If you would like to know more details about our services, please contact us directly or send an email with your specific requirements.
References
- Zhang, Xiaoqing, et al. "Circular RNAs and esophageal cancer." Cancer cell international 20 (2020): 1-9.
- Bach, Duc-Hiep, Sang Kook Lee, and Anil K. Sood. "Circular RNAs in cancer." Molecular Therapy-Nucleic Acids 16 (2019): 118-129.
