Custom Mitochondrial Targeting mRNA Synthesis

Introduction of Mitochondrial Targeting peptides

Mitochondria-targeting peptides (Mitp or Mito) have specific amino acid sequences designed to target carrier molecules or drugs to the mitochondria within cells. Mitochondrial Targeting mRNA is a technology that transports nuclear messenger RNA to the mitochondrial surface and imports newly generated proteins into the mitochondrial matrix, restoring mitochondrial function. The mitochondrial targeting sequence (MTS) used commonly is the N-terminal 23 amino acids of cytochrome oxidase subunit 8 precursor protein (MSVLTPLLLRGLTGSARRLPVPR). The high affinity of Mito include:

  • They contain specific amino acid sequences with a high affinity for specific proteins or structures on the mitochondrial membrane.
  • Since mitochondrial membranes are negatively charged, they often carry a positive charge, facilitating interactions between peptides and mitochondrial membranes.
  • They can be easily engineered to be fused to specific molecules or to confer mitochondrial targeting properties.

Application of Mito-mRNA

Mito-mRNA has a wide range of applications, including the treatment of mitochondrial genetic diseases, cancer treatment and immunotherapy, oxidative stress management, and mitochondrial-related genetic editing. In gene editing, the combination of Mito and gene editing tools like Cas9 allows for the modification of mitochondrial DNA (mtDNA), which is critical for the investigation and treatment of mitochondrial function disorders. Figure 1 displays an example of CRISPR/Cas9 delivery to the mitochondria. PNPase-dependent RNA import can be employed to carry hybrid sgRNA into the mitochondria and simultaneously express Cas9 in the mitochondria by delivering Cas9 mRNA with a mitochondria localization signal. In mitochondria, Cas9 forms a complex with the RP loop sgRNA, causing mtDNA cleavage at specified sequences.

Graphical summary of CRISPR/Cas9 delivery into the mitochondriaFig.1 Graphical summary of CRISPR/Cas9 delivery into the mitochondria.1

Our Mito-mRNA Synthesis Service

Replenishing nucleic acids into mitochondria is a straightforward approach to addressing mitochondrial disorders. Mitochondrial targeting strategies can be utilized to deliver mRNA to mitochondria, allowing for the correction of genetic errors and damage, as well as the therapy of mitochondrial disorders. Creative Biolabs provides various types of mRNA synthesis services and mRNA quality control services. We can supply our customers with high-yield IVT Mito-mRNA synthesis services. The advantages of our service include but are not limited to:

  • Efficiency: Provide an efficient in vitro transcription system to ensure high-yield Mito-mRNA production.
  • Purity and Quality: Supply highly pure and quality-controlled Mito-mRNA suitable for various experiments and applications.
  • Professional Support: Offer professional technical support and consultation to ensure the success of clients' experiments.

If you have any specific requirements about Mito-mRNA synthesis, please feel free to contact us for more information.

Reference

  1. Hussain, Syed-Rehan A., et al. "Adapting CRISPR/Cas9 system for targeting mitochondrial genome." Frontiers in Genetics 12 (2021): 627050.
For research use only. Not intended for any clinical use.