Ebola Virus
Pseudotyping lentivirus vector has been proved to be a useful strategy for gene therapy. Currently, a wide variety of heterologous viral envelope glycoproteins have successfully been used to pseudotype lentiviral vectors, including those from vesiculoviruses, hepadnaviruses, flaviviruses, baculovirus, alphaviruses, and filoviruses. Working in the field of gene therapy for many years, Creative Biolabs is capable of offering high-quality lentiviral vector construction and optimization service for global customers. Here, we are happy to help customer construct lentiviral vector harboring Ebola virus-derived glycoprotein.
Ebola Virus
Ebola viruses are the causative agent of a severe hemorrhagic fever disease associated with mortality rates of up to 88% in humans. As a nonsegmented, negative-sense RNA virus of the Filovirus family, it has a unique filamentous morphology with a uniform diameter of 80 nm and variable length of up to 14 μm. Infection of Ebola virus can be initiated by virus entry into the body through mucosal sites or by direct contact (through open wounds). Once inside the body, the primary target cells for Ebola are thought to be dendritic cells and macrophages. These infected cells then carry the virus to the primary organs of viral replication, including the liver, spleen, lung, and lymph nodes.
Figure 1. Structure of Ebola virus.
Pseudotypes Based on Ebola-derived GPs
Ebola virus envelope glycoprotein (Ebo-GP) has a molecular mass of approximately 140 kDa. As the sole viral spike protein, Ebo-GP is presumed to be responsible for mediating viral entry into target cells. In recent years, vectors derived from lentiviral offer particularly flexible properties in gene transfer applications. Given the numerous possible associations of various viral surface glycoproteins (determining cell tropism) with different types of lentiviral cores, the boundary of the host immune system and their host range have been extremely broadened. Indeed, vesicular stomatitis virus (VSV) pseudotypes based on Ebola-derived GPs have been widely employed in studies focused on investigating mechanisms of Ebola virus entry into target cells, in the screening of antiviral compound libraries, for the development of tests aimed at the identification of neutralizing antibodies, and as vaccine vectors.
Services
Working in the field of gene therapy for years, Creative Biolabs has constructed and optimized numerous glycoprotein based lentiviral vectors for global customers. In addition to VSV pseudotypes, we are committed to developing and optimizing more and more specific virus for glycoprotein pseudotypes. Our professional scientists will try their best to offer high-quality lentiviral vector construction services in the most high-quality and cost-effective way to make your project a success.
For more detailed information, please feel free to contact us or directly send us a quote.
