Hepacivirus

Glycoprotein is one of the major components of human pathogenic viruses. They have been shown to play an important role in infection and immunity. The development of novel diagnostic antibodies against these antigenic viral glycoproteins has also demonstrated potential in the field of infectious disease treatment. Since viral envelope glycoproteins are a key consideration in the interaction of receptors with neutralizing antibodies, it is necessary to design glycoprotein optimization strategies.

Creative Biolabs has a team of professional gene therapy scientists who are experienced in the optimization of viral envelope glycoproteins. Our advanced variant optimization technology platform enables the optimized LV to remain active to meet the customer's research needs.

Introduction of Hepacivirus

Here, we focus on an important class of LV pseudotypes that target CD81⁺ cells - Hepacivirus.

Hepacivirus is a genus of the sense single-stranded RNA virus in the Flaviviridae family. The most important member of the Hepacivirus family is the hepatitis C virus (HCV), the only known natural host of humans associated with hepatitis and hepatocellular carcinoma. HCV is primarily transmitted through the bloodstream of chronic carriers, such as hemodialysis patients, hemophiliacs, infected mothers, injecting drug users (injecting drug users), and high-risk sexual behavior. Cases of acute HCV (approximately 85%) usually develop into chronic hepatitis. Untreated chronic hepatitis can further lead to serious sequelae such as cirrhosis and hepatocellular carcinoma.

Figure 1. Hepacivirus. (From ViralZone)

Pseudotyping of Lentiviral Vector with Hepacivirus

Pseudotyped viruses play an important role in the study of virus entry process, receptor identification, neutralizing antibody detection, vaccine evaluation and the like. Creative Biolabs provides pseudotyping services of lentiviral vector for targeting CD81+ cells using envelope proteins derived from hepacivirus. Pseudotype virus is produced by a combination of exogenous viral envelope proteins, which has been shown to have several compelling advantages compared to traditional lentiviruses:

After glycoprotein optimization of the virus you are interested, Creative Biolabs performs binding assays to ensure the effect of glycosylation of the protein on viral function. Based on structural optimization, we also offer a range of downstream services such as Potency Tests, Safety and Toxicology Analysis, Delivery Systems Development, etc. to meet your needs.

Please feel free to contact us and our experienced technicians will give you the most detailed answers to your questions.

References

1. Azoun, S.B., et al. (2016). Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris. Microbial Biotechnology. 9.
2. Clarke, E.C., et al. (2017). Production and Purification of Filovirus Glycoproteins in Insect and Mammalian Cell Lines. Scientific Reports. 7(1):15091.