With a state of art R&D facility and in-house expertise, scientists at Creative Biolabs are able to engineer liver cancer cell MHCC97L utilizing several strategies, turning cancer cells against themselves.

Engineering MHCC97L Cell for Cancer Therapy

Engineering patients' cancer cells or cancer cells grown in a lab is a promising method for cancer immunotherapy and vaccine research. They can cause an immune response against features of cancer cells that express a large number and variety of tumor-specific antigens. Cytokine gene-modified tumor cells can be used as vaccines in the immunotherapy of cancer. Due to their viability and easy availability, cell lines are used for antibody production, vaccine development, and cancer cell studies, among others. Creative Biolabs provides services to develop self-destructive human liver cancer cell lines (such as MHCC97L) by overexpression of cytokines or targeting intracellular signaling and transcriptional regulators. We are dedicated to engineering MHCC97L cells utilizing several strategies, turning cancer cells against themselves.

MHCC97L Cell Line

The MHCC97 is a human hepatocellular carcinoma (HCC) cell line. The cell line is derived from an intrahepatic metastatic lesion of a male Chinese HCC patient with a hepatitis B virus (HBV) infection background. It has been established from the subcutaneous xenograft of a metastatic model of human HCC in nude mice (LCI-D20). MHCC97 is a heterogeneous population, consisting of subpopulations with different metastatic potentials. Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent MHCC97 cell line. Compared with MHCC97-H cells, which exhibit a highly invasive phenotype, the MHCC97-L cell line is characterized by larger cell volume and slower growth, invasion, and metastasis.

Engineering MHCC97L Cell Service

Cytokines as a key component of cancer-related inflammation and play pleiotropic functions. A surprisingly large number of cytokines possess antitumor activity (IL-2, IFN-γ, GM-CSF, etc). We provide services to engineer MHCC97L cells for self-destruction by inserting and expressing cytokine genes in tumor cells. Available gene expression and delivery technologies include viral transduction (retrovirus, lentivirus, adenovirus, adeno-associated virus), non-viral delivery systems, and CRISPRs. Our experienced cell engineering team will help determine the best-suited technology based on your project.

In addition to cytokine overexpression, other strategies include engineering cancer cells with target-specific aptamer, synthetic immunomodulatory gene circuits, targeted siRNA delivery, etc. Our team can help to create construct and engineer the cell line. Based on your project needs, our solutions can further custom tune-up and optimize.

One-stop Services

Creative Biolabs provides a powerful and clinically-promising platform with which to engineer cancer cells for self-destruction. Our one-stop services include strategy design, cell engineering, and in vitro & ex vivo assessment. Please feel free to contact our dedicated technical support team with any questions, or get started on your services project.

Reference

1. Huang, H.; et al. Oncolytic adenovirus programmed by synthetic gene circuit for cancer immunotherapy. Nature communications. 2019, 10(1): 1-15.

For Research Use Only | Not For Clinical Use