SLC9A1 Membrane Protein Introduction

Introduction of SLC9A1

Solute carrier family 9A1 (SLC9A1) is also known as sodium/hydrogen exchanger 1 or sodium-hydrogen antiporter 1 (NHE-1). It is encoded by the SLC9A1 gene in humans. It’s ubiquitous expressed in stomach, salivary gland and 23 other tissues. SLC9A1 belongs to the solute carrier family 9, and functions as a Na⁺/H⁺ antiporter. It plays a critical role in regulating pH homeostasis, cell volume, and cell migration. It might also be involved in tumor growth.

Basic Information of SLC9A1
Protein Name	Sodium/hydrogen exchanger 1
Gene Name	SLC9A1
Aliases	APNH1, NHE1
Organism	Homo sapiens (Human)
UniProt ID	P19634
Transmembrane Times	12
Length (aa)	815
Sequence	MVLRSGICGLSPHRIFPSLLVVVALVGLLPVLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYEHVGIVDIFLGFLSFFVVALGGVLVGVVYGVIAAFTSRFTSHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPSERILPALSKDKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ

Function of SLC9A1 Membrane Protein

SLC9A1 (also known as NHE1) belongs to proton exchangers and transporters family. It is involved in pH homeostasis and associated with developments of different malignancies. Activation of SLC9A1 can be able to avoid intracellular acidification and then protect the cells against apoptosis. Suppressions of SLC9A1 can decrease intracellular pH and lead to apoptosis. SLC9A1 is regulated at the post-translational level and regulated by different binding proteins. For example, SLC9A1 can interact with NCX1 and CaM to mediate the effects of IL6 on human hepatocellular carcinoma. Except for the intracellular pH regulation, SLC9A1 is also involved in different normal and pathological cellular processes. For example, it’s involved in cell cycle regulation, cell movement, and apoptosis. These may happen in heart disease and malignancies. In cancers, SLC9A1 acts as a critical role to maintain a reversed proton gradient in cancer cells. This function is particularly important in cell division and proliferation.

Fig.1 Structure of SLC9A1.

Application of SLC9A1 Membrane Protein in Literature

Abdelazeem K.N.M., et al. Negative effect of ellagic acid on cytosolic pH regulation and glycolytic flux in human Endometrial Cancer Cells. Cell Physiol Biochem. 2017, 41(6): 2374-2382. PubMed ID: 28467979

This article indicates that SLC9A1 is involved in reprogramming and growth inhibition of tumor cells through modulating Na⁺/H⁺ exchanger activity, lactate release, glucose uptake in endometrial cancer cells.
Zhu W., et al. Glioma-mediated microglial activation promotes glioma proliferation and migration: roles of Na⁺/H⁺ exchanger isoform 1. Carcinogenesis. 2016, 37(9): 839-851. PubMed ID: 27287871

This article demonstrates that SLC9A1 plays a critical role in microglia-glioma interactions. Suppression of SLC9A1 can be a novel glioma therapeutic strategy.
Xu J., et al. Na⁺/H⁺ exchanger 1, Na⁺/Ca²⁺ exchanger 1 and calmodulin complex regulates interleukin 6-mediated cellular behavior of human hepatocellular carcinoma. Carcinogenesis. 2016, 37(3): 290-300. PubMed ID: 26775040

This article shows that SLC9A1 can interact with NCX1 and CaM to mediate the effects of IL6 on human hepatocellular carcinoma.
Xu L., et al. Nucleoporin 35 regulates cardiomyocyte pH homeostasis by controlling Na⁺/H⁺ exchanger-1 expression. J Mol Cell Biol. 2015, 7(5): 476-85. PubMed ID: 26260029

This article indicates that Nup35 can effectively regulate cardiomyocyte pH homeostasis by controlling SLC9A1 expression. This finding may provide insight into the therapeutic strategy for ischemic cardiac diseases.
Parker M.D., et al. Na⁺/H⁺ exchanger-1 (NHE1) regulation in kidney proximal tubule. Cell Mol Life Sci. 2015, 72(11): 2061-74. PubMed ID: 25680790

This article demonstrates that SLC9A1 plays an important role as a kidney cell survival factor in kidney proximal tubule.

SLC9A1 Preparation Options

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