SLC9A2 Membrane Protein Introduction

Introduction of SLC9A2

Solute carrier family 9A2 (SLC9A2) is also known as sodium-hydrogen antiporter 2 (NHE-2) or sodium/hydrogen exchanger 2. It is encoded by the SLC9A2 gene in humans. It’s biased expressed in stomach, colon and 10 other tissues. It belongs to the solute carrier family 9, and functions as a Na⁺/H⁺ antiporter. SLC9A2 is localized to the apical membrane and associated with apical absorption of sodium.

Basic Information of SLC9A2
Protein Name	Sodium/hydrogen exchanger 2
Gene Name	SLC9A2
Aliases	NHE2
Organism	Homo sapiens (Human)
UniProt ID	Q9UBY0
Transmembrane Times	10
Length (aa)	812
Sequence	MEPLGNWRSLRAPLPPMLLLLLLQVAGPVGALAETLLNAPRAMGTSSSPPSPASVVAPGTTLFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTRPFFENIGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLLFGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIETIDVFAGIANFFVVGIGGVLIGIFLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLMWRALGVFVLTQVINRFRTIPLTFKDQFIIAYGGLRGAICFALVFLLPAAVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIYCRLFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIEMAETGMISTVPTFASLNDCREEKIRKVTSSETDEIRELLSRNLYQIRQRTLSYNRHSLTADTSERQAKEILIRRRHSLRESIRKDSSLNREHRASTSTSRYLSLPKNTKLPEKLQKRRTISIADGNSSDSDADAGTTVLNLQPRARRFLPEQFSKKSPQSYKMEWKNEVDVDSGRDMPSTPPTPHSREKGTQTSGLLQQPLLSKDQSGSEREDSLTEGIPPKPPPRLVWRASEPGSRKARFGSEKP

Function of SLC9A2 Membrane Protein

SLC9A2 is also known as sodium/hydrogen exchanger 2 (NHE2) or sodium-hydrogen antiporter 2, which belongs to proton exchangers and transporters. Sodium-hydrogen exchanger (NHE) protein family mediates the sodium-ion transport by exchanging intracellular hydrogen ions to external sodium ions and plays an important role in cell pH regulation and cell volume. SLC9A2 is localized to the apical membrane and associated with apical absorption of sodium. As a member of SLC9A family, SLC9A2 might be affected by clinical exposure to NSAIDs just like other family members and this will lead to transport modulation and barrier function. While there are different activities between the isoforms, SLC9A2 activation can increase the migratory velocity of RGM1 cells, but SLC9A1 activation can inhibit migratory velocity; SLC9A2 is necessary for mouse gastric epithelial restitution, while SLC9A1 is not essential. SLC9A2 can mediate butyrate-dependent sodium absorption in dextran sulfate sodium-induced colitis. It’s also regulated at the post-translational level, and regulated by different binding proteins.

Fig.1 Topology of SLC9A family. (Hendus-Altenburger, 2014)

Application of SLC9A2 Membrane Protein in Literature

Paehler Vor der Nolte A., et al. Na⁺ /H⁺ exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells. J Cell Physiol. 2017, 232(7): 1669-1680. PubMed ID: 28019659

This article indicates that SLC9A2 plays an opposite activity compared with SLC9A1. SLC9A1 can increase the migratory velocity of RGM1 cells, while SLC9A2 inhibits this activity.
Rajendran V.M., et al. Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na⁺ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis. J Biol Chem. 2015, 290(42): 25487-96. PubMed ID: 26350456

This article demonstrates that SLC9A2 can mediate butyrate-dependent sodium absorption in DSS induced colitis. This finding provides insight that SLC9A2 can be as a therapeutic target.
Roginiel A.C., et al. Effect of NSAIDs on Na⁺/H⁺ exchanger activity in rat colonic crypts. Am J Physiol Cell Physiol. 2013, 305(5): C512-8. PubMed ID: 23739181

This article shows that NHE isoforms including NHE2 might be affected by clinical exposure to NSAIDs, and this will lead to transport modulation and barrier function.
Muthusamy S., et al. PKCδ-dependent activation of ERK1/2 leads to upregulation of the human NHE2 transcriptional activity in intestinal epithelial cell line C2BBe1. Am J Physiol Gastrointest Liver Physiol. 2012, 302(3): G317-25. PubMed ID: 22052014

This article demonstrates that NHE2 expression can be upregulated by PKCδ dependent activation of ERK1/2 in the intestinal epithelial cell line.
Xue L., et al. Trefoil factor 2 requires Na/H exchanger 2 activity to enhance mouse gastric epithelial repair. J Biol Chem. 2012, 286(44): 38375-82. PubMed ID: 21900251

This article demonstrates that SLC9A2 is necessary for mouse gastric epithelial restitution, while SLC9A1 is not necessary.

SLC9A2 Preparation Options

With the superior Magic™ membrane protein production platform, we are able to present target membrane protein in multiple active formats. Our professional senior scientists are committed to providing the most suitable method for our clients and maximize the success of your projects. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC9A2 antibody development services.

Creative Biolabs offers high-quality membrane protein preparation services to facilitate the projects of worldwide customers. With years of extensive experience in the field of membrane protein preparation, we have successfully established an advance Magic™ membrane protein platform which enables us to provide a series of membrane protein preparation services. For more detailed information, please feel free to contact us.

Reference

Hendus-Altenburger R, et al. (2014). Structural dynamics and regulation of the mammalian SLC9A family of Na⁺/H⁺ exchangers. Curr Top Membr. 73:69-148.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: