SLC9A6 Membrane Protein Introduction

Introduction of SLC9A6

Solute carrier family 9A6 (SLC9A6) is also known as sodium-hydrogen antiporter 6 (NHE-6) or sodium/hydrogen exchanger 6. SLC9A6 is encoded by the SLC9A6 gene in humans, which is ubiquitously expressed in the brain, adrenal, and 23 other tissues. The SLC9A6 gene is conserved in Rhesus monkey, chicken, chimpanzee, dog, rat, zebrafish, fruit fly, mouse, mosquito, cow, C.elegans, and frog. It belongs to the solute carrier family 9, and functions as a Na⁺/H⁺ antiporter. SLC9A6 is documented to be involved in pH homeostasis, and signal transduction.

Function of SLC9A6 Membrane Protein

As a proton exchanger and transporter, SLC9A6 mediates the sodium-ion transport by exchanging intracellular hydrogen ions to external sodium ions and plays an important role in cell pH regulation and cell volume. As a member of SLC9A family, SLC9A6 is associated with Nef internalization into M2 macrophages. The relocalization of SLC9A6 from endosomes to plasma membrane is related to endosome hyperacidification. In addition, SLC9A6 is identified in the choroid plexus and it may be involved in pH modulation of the CSF. SLC9A6 expression is down-regulated in Caco-2 cells via the mechanisms of rotavirus-induced diarrhea. Moreover, SLC9A6 is associated with recycling endosomal function and neuronal degeneration and cell death. SLC9A6 is also functionally associated with Alzheimer disease via endosomal pH modulation and amyloid precursor protein. Furthermore, SLC9A6 plays a critical role in endosome hyperacidification and chemoresistance.

Fig.1 Model for endosomal pH regulation of Aβ production by NHE6. (Prasad, 2015)

Application of SLC9A6 Membrane Protein in Literature

1. Damkier H.H., et al. The murine choroid plexus epithelium expresses the 2Cl^-/H⁺ exchanger ClC-7 and Na⁺/H⁺ exchanger NHE6 in the luminal membrane domain. Am J Physiol Cell Physiol. 2018, 314(4): C439-C448. PubMed ID: 29351414
   
   

   This article indicates that SLC9A6 is identified in the choroid plexus for the first time, and it may be involved in pH modulation of the CSF.

2. Lucien F., et al. Hypoxia-induced mobilization of NHE6 to the plasma membrane triggers endosome hyperacidification and chemoresistance. Nat Commun. 2017, 8: 15884. PubMed ID: 28635961
   
   

   This article shows that NHE6 plays a critical role in endosome hyperacidification and chemoresistance.

3. Chen H., et al. Human rotavirus strain Wa downregulates NHE1 and NHE6 expressions in rotavirus-infected Caco-2 cells. Virus Genes. 2017, 53(3): 367-376. PubMed ID: 28289928
   
   

   This article indicates that SLC9A6 expression is down-regulated in Caco-2 cells via the mechanisms of rotavirus-induced diarrhea.

4. Ilie A., et al. A Christianson syndrome-linked deletion mutation (∆(287)ES(288)) in SLC9A6 disrupts recycling endosomal function and elicits neurodegeneration and cell death. Mol Neurodegener. 2016, 11(1): 63. PubMed ID: 27590723
   
   

   This article shows that functional deficiency caused by the mutation in SLC9A6 is associated with recycling endosomal function and neuronal degeneration and cell death.

5. Prasad H. and Rao R. The Na⁺/H⁺ exchanger NHE6 modulates endosomal pH to control processing of amyloid precursor protein in a cell culture model of Alzheimer disease. J Biol Chem. 2015, 290(9): 5311-27. PubMed ID: 25561733
   
   

   This article demonstrates that SLC9A6 is functionally associated with Alzheimer disease via endosomal pH modulation and amyloid precursor protein.

SLC9A6 Preparation Options

Leveraging our advanced Magic™ membrane protein production platform, we are able to provide membrane protein preparation service and offer target membrane protein in multiple active formats. Our professional senior scientists aim to provide the products with superior quality to our customers and are dedicated to facilitating the success of your projects. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-SLC9A6 antibody development services.

• Magic™ Membrane Protein Production Platform
• Magic™ Anti-Membrane Protein Antibody Discovery Platform

Creative Biolabs is committed to offering the most superior products to our clients to best maximize the success of their projects.  With years of extensive experience in the field of membrane protein preparation, we have won a good reputation and established a perfect transmembrane protein platform to facilitate a wide range of projects for numerous clients. For more detailed information, please don’t hesitate to contact us.

Reference

1. Prasad H and Rao R. (2015). The Na⁺/H⁺ exchanger NHE6 modulates endosomal pH to control processing of amyloid precursor protein in a cell culture model of Alzheimer disease. J Biol Chem. 290(9): 5311-27.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.