Custom iPSC-derived Macrophage Service

Creative Biolabs is a leading service provider that focuses on inducible pluripotent stem cells (iPSCs)-derived cell therapy services. Currently, various platforms have been established for analyzing the function of iPSC-derived macrophage in developing novel stem cell therapy. By combining cutting edge technologies with proprietary innovations, we also provide broadly applicable iPSC-derived macrophage models for a wide range of disease types.

Introduction of iPSC-derived Macrophages

iPSCs, a member of stem cell family, play an important role in generating a number of specific immune cells for different types of disease treatments, especially for various cancers. In the past few years, a wide variety of techniques have been developed to produce iPSCs, including different vectors. Previous studies have demonstrated that four reprogramming factors, Oct4, Sox2, Klf4, and c-Myc, have been commonly used for mediating vector-based delivery for iPSC generation, also known as iPSC reprogramming factors delivery by episomal vectors. Meanwhile, pilot studies have indicated that iPSC can be derived from immune cells, such as macrophages, to develop new stem cell therapy for treating various genetic diseases. Moreover, recent researchers have revealed that iPSC-derived macrophages are critical for establishing macrophage polarization models and for analyzing the function of macrophages in human genetic disorders.

Fig.1 Custom iPSC-derived Macrophage Services.

Custom iPSC-derived Macrophage Service

At Creative Biolabs, we deeply understand the requirement of our clients in iPSC-derived macrophage services. We are always dedicated to developing and improving our skills to make custom services for your target of interest. Up to now, we have generated a full range of iPSC-derived macrophage assays to offer a comprehensive iPSC-based solution for disease immunotherapy, such as differentiation services of iPSCs to macrophages, functional analysis of iPSC-derived macrophages, transcriptome analysis of iPSC-derived macrophages, iPSC-derived macrophage polarization assays, and disease modeling of iPSC-derived macrophages. Based on our advanced technologies, iPSC-derived macrophages can be regarded as unlimited genotype-specific cells to permit deeper insights into the relationship of the function of macrophages and plenty of human diseases.

Macrophages play a crucial role in the immune response and are key players in inflammation and tissue repair processes. By using iPSC technology, we are able to provide a consistent and scalable source of macrophages for a variety of research applications.

• Researchers can customize their order by choosing specific donor iPSC lines, differentiation protocols, and cell culture conditions to suit their specific research needs.
• The custom iPSC-derived macrophage service also includes comprehensive characterization of the resulting macrophages, including functional assays such as phagocytosis, cytokine production, and migration ability.

Our custom service is designed to provide researchers with a reliable and convenient source of high-quality macrophages for a variety of research applications, including drug discovery, immunology, and regenerative medicine.

Features of Our Services

• Rapid, high-throughput generation of iPSC-derived macrophages
• The morphological, functional, and transcriptome profiles of iPSC-derived macrophages
• Functional disease modeling in iPSC-derived macrophages
• Novel insights into macrophage inflammatory response
• Flexible, cost-effective, and time-saving process

Fig.2 Anticancer therapy with iPSC-derived macrophages producing interferon β. ¹

With the help of abundant experience and professional scientists, Creative Biolabs is committed to guiding global customers to a series of custom iPSC-derived macrophage services. Our laboratory services have many advanced chemical and biological technologies to support customer's needs in the fast-growing iPSC-derived cell therapy research. If you are interested in our services, please feel free to contact us or send us an inquiry.

Published Data

Below are the findings presented in the article related to ipsc-derived macrophage.

Simon Gutbier et al. described an improved method for the use of iPSC for the high-yield, large-scale production of cells resembling tissue-resident macrophages. They thoroughly characterized the iPSC-derived macrophage-like cells to confirm their cell identity and thus their suitability for drug screening purposes.

The results showed that these iPSC-derived macrophages shared strong cellular identity with primary macrophages and recapitulated key functional characteristics, including cytokine release, phagocytosis, and chemotaxis. In addition, they demonstrated that genetic modifications can be easily introduced at the macrophage-like progenitor cell stage to probe drug target-related pathways.

Fig. 3 Comparison of functionality of iPSC-derived and CD14+ cells derived from PBMCS.²

FAQs

• Q: Can you customize the phenotype or polarization state of iPSC-derived macrophages?
  A: Yes, we offer complete flexibility in customizing the phenotype or polarization state of the iPSC-derived macrophages according to your research needs. Whether you require M1, M2, or alternative macrophage states, we can fine-tune the differentiation protocols using specific cytokines and growth factors. Additionally, we can incorporate genetic modifications or apply environmental conditions that mirror your experimental requirements, ensuring that the macrophages match your intended model.
• Q: What types of genetic modifications can be incorporated during the differentiation process?
  A: Our service offers a wide range of genetic modification options, including CRISPR/Cas9-mediated gene editing, overexpression or knockdown of target genes, and the introduction of fluorescent reporters or tags. These modifications can be introduced at different stages of the differentiation process, allowing us to tailor the macrophages to your specific experimental goals. We also perform comprehensive validation to ensure that the modifications are stable and consistent.
• Q: How scalable is your iPSC-derived macrophage production? Can you meet large-scale research needs?
  A: Our service is fully scalable to accommodate both small pilot studies and large-scale research projects. We can generate batches of iPSC-derived macrophages ranging from a few million cells to several billion cells, depending on your requirements. We utilize high-efficiency bioreactors and optimized differentiation protocols to ensure consistency and scalability without compromising quality.
• Q: How long does it take to produce a batch of iPSC-derived macrophages?
  A: The timeline for generating a custom batch of iPSC-derived macrophages can vary depending on the complexity of the project. Typically, the differentiation process takes 4-6 weeks, with additional time needed if genetic modifications or extensive customization is required. We work closely with you to establish a detailed project timeline and keep you updated throughout the process.
• Q: Do you offer characterization data with the final product?
  A: Yes, we provide comprehensive characterization data along with the final batch of iPSC-derived macrophages. This typically includes flow cytometry data, immunostaining results for key macrophage markers, functional assay outcomes, and any additional characterization relevant to your project, such as cytokine profiling. We aim to deliver all necessary data that validates the functionality and identity of the macrophages for your research.

Scientific Resources

iPSC Differentiation

iPSC for Cell Therapy

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References

1. Senju, Satoru, et al. "Application of iPS cell-derived macrophages to cancer therapy." Oncoimmunology 3.3 (2014): e27927.
2. Gutbier, Simon, et al. "Large-scale production of human iPSC-derived macrophages for drug screening." International journal of molecular sciences 21.13 (2020): 4808.

For Research Use Only. Not For Clinical Use.