TAS2R43 Membrane Protein Introduction

Introduction of TAS2R43

TAS2R43 is a member of the G-protein coupled receptor T2R family. The perception of bitter taste is mediated by TAS2Rs, which is a family of G protein-coupled receptors (GPCRs). TAS2Rs are mainly expressed in bitter taste cells on our tongue and transduce intracellular signals through the activation of intracellular heterotrimeric G proteins. These receptors recognize bitter compounds various in chemical structure.

Function ofTAS2R43 Membrane Protein

TAS2R receptors are coupled to G proteins and second messenger pathways and are mainly expressed at the surface of taste receptor cells. We humans distinguish different tastes through those receptors. TAS2R43 is a member of TAS2R family. It has bitter taste receptor activity. At the same time, it also has G-protein coupled receptor activity. The main role of TAS2R43 is transducing signal of bitness to intracellular, and then to brain by nervous system, and finally, we feel bitter. From the predicted results performed using TOPCONS, we can see TAS2R43 has 7 transmembrane regions and 2 glycosylation sites (161 and 176) and some other particular sites marked with different colors (Fig. 1). By the way, TAS2R43 as a membrane protein is difficult to be gotten tertiary structure. So far, the 3D structure of it hasn't been determined.

Fig.1 Secondary Structure Prediction of TAS2R43 with the Position of the Transmembrane Region Performed using TOPCONS. (Ayana, 2012)

Application of TAS2R43 Membrane Protein in Literature

1. Suess B., et al. The odorant (R)-citronellal attenuates caffeine bitterness by inhibiting the bitter receptors TAS2R43 and TAS2R46. J Agric Food Chem. 2018, 66(10): 2301-2311. PubMed ID: 27569025
   
   

   This article reveals (R)-citronellal to completely block caffeine-induced calcium signals in TAS2R43-expressing cells, and, to a lesser extent, in TAS2R46-expressing cells and has proved 3-methyl-branched aliphatic aldehydes with a carbon chain of ≥4 C atoms to be the best TAS2R43 antagonists by further structure/activity studies.

2. Sandau M.M., et al. A functional comparison of the domestic cat bitter receptors Tas2r38 and Tas2r43 with their human orthologs. BMC Neurosci. 2015, 16: 33. PubMed ID: 26037485
   
   

   This article demonstrates that the response profiles of the human bitter receptors Tas2r43 and Tas2r38 are different from those of their orthologous cat receptors. Results with cat Tas2r38 also demonstrate that additional residues beyond those classically associated with PROP sensitivity in humans influence the sensitivity to PROP and PTC. Functional studies of the human bitter receptor family have contributed to the development of food and medicinal products.

3. Pirastu N., et al. Association analysis of bitter receptor genes in five isolated populations identifies a significant correlation between TAS2R43 variants and coffee liking. PLoS One. 2014, 9(3): e92065. PubMed ID: 24647340
   
   

   This article finds a significant association between differences in caffeine perception and the H212R variant but not with the W35S (both variants have been shown to greatly reduce in vitro protein activity), which suggests that the effect of the TAS2R43 gene on coffee liking mostly relate to caffeine and in particular the H212R variant.

4. Wooding S.P., et al. Association of a bitter taste receptor mutation with Balkan Endemic Nephropathy (BEN). BMC Med Genet. 2012, 13: 96. PubMed ID: 23050764
   
   

   This article reveals that BEN is positively related with TAS2R43-W35/H212, an allele previously shown to confer elevated bitter sensitivity to aristolochic acid, which may cause aversion but might also affect absorption, altering toxin activation.

5. Liszt K.I., et al. Caffeine induces gastric acid secretion via bitter taste signaling in gastric parietal cells. Proc Natl Acad Sci U S A. 2017,114(30): E6260-E6269. PubMed ID: 28696284
   
   

   This article reveals that the caffeine-evoked effect is shown to depend on one of its cognate receptor TAS2R43.

TAS2R43 Preparation Options

In order to provide high-quality membrane protein preparation service, we have developed versatile Magic™ membrane protein production platform. Our experienced scientists will do their best to help you find a perfect match to meet your needs. Aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-TAS2R43 antibody development services.

• Magic™ Membrane Protein Production Platform
• Magic™ Anti-Membrane Protein Antibody Discovery Platform

Creative Biolabs is dedicated to providing first-class membrane protein production service using a variety of strategies. Based on our leading-edge platform, we have successfully produced, purified, stabilized and characterized many challenging membrane proteins for global customers. If you are interested in the services, please contact us for more information.

Reference

1. Ayana Wiener, et al. (2012). A database of bitter compounds. Nucleic Acids Res. 40(Database issue), D413-419.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.