Hamster Hybridoma based Antibody Discovery Service

Creative Biolabs has extensive experience in producing monoclonal antibodies in hamsters. The use of a hamster as the host animal offers a unique species alternative in areas where mouse and rat antibodies may not work well. We can employ up to 10 hamsters for each antigen. Immune responses are elicited using immunogens such as transfected cell lines, DNA, recombinant proteins, native proteins, peptides, or haptens conjugated to carrier molecules.

Overview of Hamster Hybridoma

Fig.2 Pearl Winter White Russian Dwarf Hamster. (https://en.wikipedia.org/wiki/Winter_white_dwarf_hamster#/media/File:Pearl_Winter_White_Russian_Dwarf_Hamster_-_Front.jpg)

The evolutionary proximity of rats and mice may limit antibody responses. Both species belong to the Muridae Murinae and thus likely share many conserved antigenic structures. To avoid this limitation, evolutionarily more distant hamsters were immunized hybridoma cells secreting hamster Monoclonal antibodies (mAbs) were obtained by fusing mouse CTLS and their splenocytes with mouse myeloma cells. The hamster hybridoma can be used for the study of the hamster alloantigens, the cloning of the hamster lg gene, and the determination of the hamster lg subclasses.

Services

Mice CTL immunization of hamsters was performed with hybrid cells between mouse myeloma cells and Syrian or Armenian hamster spleen cells that produced secreted specific antibodies. mAbs were screened by indirect binding assay and inhibition of CTL killing effect. In the previous project, the hamster hybridoma cell line remained stable after the first cloning. Subclones were 100% active, indicating that these hybridomas did not require multiple cloning cycles to stabilize. Antibodies derived from hamster hybridomas may reveal antigenic determinants that are conserved between mouse and rat, but immunogenic in mouse-hamster combinations. Creative Biolabs provides one-stop hamster hybridoma development services to assist your drug discovery program.

Addition services also can be choosen for the hamster hybridomas selected, such as amino acid sequencing, production of serological reagents, definition of the hamster lg subclasses, and cloning of the hamster lg gene.

Applications of Hamster Hybridoma

Our high-affinity hamster antibodies are also suitable for common research applications, including chromatin immunoprecipitation, ELISA, fluorophore-linked immunosorbent assay, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and western blot.

Fig.1 Hamster antibodies fight viral infection.¹

Features of Hamster Hybridoma

High affinity and specificity
Cross-reactive antibodies that recognize human proteins, mouse, and rat homologs
New epitope recognition
Good resources for producing IgG and IgM
Capable of producing a large number of biologically active antibodies

If you are interested in our hamster hybridoma production service or have a project requirement, you are always welcome to contact our experts to further discuss this interesting topic.

Reference

Ye, Zi-Wei, et al. "Cross-variant protection against SARS-CoV-2 infection in hamsters immunized with monovalent and bivalent inactivated vaccines." International Journal of Biological Sciences18.12 (2022): 4781.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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