The Insect Cell Extract Membrane Protein Expression System is convenient, quick, and allows both transcription and translation to occur in a single tube for the cell-free expression of membrane proteins using an extract derived from the Spodoptera frugiperda (Sf21) cell line. Membrane protein synthesis reactions are initiated via the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis and increasing protein yield.
In this kit, we recommend using pF25A/BR610 (Accession Number EU754721) or pF25K ICE T7 (Accession Number EU754722) vectors. The two vectors contain 5' and 3' untranslated (UTR) sequences from the baculovirus polyhedrin gene to enhance translation efficiency. The SgfI and PmeI restriction enzyme sites facilitate the transfer of protein-coding sequences to other vectors depending on the different expression options. The lethal barnase gene allows for the positive selection of vectors containing an insert during the cloning. The ampicillin (pF25A, Amp) and kanamycin (pF25K, Kan) resistance genes allow for selection in E. coli for choosing the positive clones.
Within the kit, it contains optimized all necessary components, listed in the table below. Simply mix these reagents with the target template and incubate them at 28-30°C in a few hours. Subsequently, analyze or purify the proteins (if needed) or store the proteins at -20°C for further processing or analysis.
Expression System | Components | Storage |
In Vitro Insect Cell Expression System | Master Mix | -70°C |
Luciferase Control DNA | -70°C | |
Nuclease-Free Water | -70°C |
Cell Extract Membrane Protein Expression System is a quick production expression system of membrane proteins in solution using biomolecular translation machinery extracted from cells of different species. Creative Biolabs now offers the most advanced insect cell-free system for membrane protein expression. Equipped with state-of-the-art technology platforms and extensive expertise, our one-stop integrated cell-free membrane protein production services can help our clients to boost their diverse research and projects. To discuss your plans for membrane protein expression, please feel free to contact us for more details.
Cat | Product Name | Expected Yield of Positive Control |
MPCFK-5 | Magic™ Cell-free Protein Expression in Insect Cell Lysate | 0.1 mg/mL |
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
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