Insect Cell Lysate based Cell-free Membrane Protein Expression Service

The Insect Cell Extract Membrane Protein Expression System is convenient, quick, and allows both transcription and translation to occur in a single tube for the cell-free expression of membrane proteins using an extract derived from the Spodoptera frugiperda (Sf21) cell line. Membrane protein synthesis reactions are initiated via the addition of a DNA template, eliminating the need for the time-consuming process of in vitro RNA synthesis and increasing protein yield.

Information About the Vector

In this kit, we recommend using pF25A/BR610 (Accession Number EU754721) or pF25K ICE T7 (Accession Number EU754722) vectors. The two vectors contain 5' and 3' untranslated (UTR) sequences from the baculovirus polyhedrin gene to enhance translation efficiency. The SgfI and PmeI restriction enzyme sites facilitate the transfer of protein-coding sequences to other vectors depending on the different expression options. The lethal barnase gene allows for the positive selection of vectors containing an insert during the cloning. The ampicillin (pF25A, Amp) and kanamycin (pF25K, Kan) resistance genes allow for selection in E. coli for choosing the positive clones.

Components List and Storage

Within the kit, it contains optimized all necessary components, listed in the table below. Simply mix these reagents with the target template and incubate them at 28-30°C in a few hours. Subsequently, analyze or purify the proteins (if needed) or store the proteins at -20°C for further processing or analysis.

Expression System	Components	Storage
In Vitro Insect Cell Expression System	Master Mix	-70°C
	Luciferase Control DNA	-70°C
	Nuclease-Free Water	-70°C

Features

Convenient, quick, single-tube, coupled transcription/translation system
Allows both transcription and translation to occur in a single reaction
Can be scaled by changing the component volumes proportionally
T7 RNA polymerase promoter
Addition of labels to the reaction
75 μg/mL of functional protein can be produced

Applications

Full-length, functional membrane protein expression
Toxic or difficult to express protein synthesis
Synthetic biology
Rapid analysis of membrane protein mutations or truncations
Protein activity and protein-protein interactions

Cell Extract Membrane Protein Expression System is a quick production expression system of membrane proteins in solution using biomolecular translation machinery extracted from cells of different species. Creative Biolabs now offers the most advanced insect cell-free system for membrane protein expression. Equipped with state-of-the-art technology platforms and extensive expertise, our one-stop integrated cell-free membrane protein production services can help our clients to boost their diverse research and projects. To discuss your plans for membrane protein expression, please feel free to contact us for more details.

Related Products

Cat	Product Name	Expected Yield of Positive Control
MPCFK-5	Magic™ Cell-free Protein Expression in Insect Cell Lysate	0.1 mg/mL

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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