HLA Neutralizing Test Service

Neutralizing antibody (NAb) is responsible for blocking the entry of a pathogen into a cell. Creative Biolabs provides human leukocyte antigen (HLA) neutralizing assay to assess the neutralizing activity of HLA antibodies against virus-carrying HLA alleles.

NAb

NAb is an antibody that is responsible for defending cells from pathogens. As part of the immune response, pathogens are triggered by both infections and vaccinations against infections. Neutralizing antibodies can result in lifelong immunity to certain infections and can be used to see if a person has developed immunity to an infection after they have recovered from it.

At present, many antibody therapies based on neutralization mechanisms have emerged for the treatment of various diseases including but not limited to infectious diseases and endocrine disorders. The neutralizing assays are specially designed to routinely validate and measure the therapeutic action of such antibodies. The role of neutralizing antibodies is as follows:

• NAbs can stop the pathogen from infecting the body by affecting the molecules on the pathogen's surface.
• In enveloped viruses (a heat-sensitive virus with a lipid membrane), NAbs can block the attachment of a virus to the cell as well as its entry into the cell.
• In non-enveloped viruses (a heat-resistant virus with no lipid membrane), NAbs can bind to the capsid protein, which is the protein shell surrounding the genetic information within a virus cell.
• NAbs can stop pathogens from changing their structure and shape, known as conformational changes, to enter and replicate within a cell.
• In bacterial infections, NAbs can block the harmful effects of toxins.
• Once a pathogen has been neutralized by a NAb, the pathogen is degraded by white blood cells, filtred by the spleen, and excreted through urine or feces.

HLA Neutralizing Assay

Creative Biolabs offers HLA antibody neutralizing assays that are specifically designed to measure antibody neutralization potential against different targets, including but not limited to virus, cytokine, and toxin.

Fig.1 Workflow of HLA neutralizing assay.

Except for HLA neutralizing assay, Creative Biolabs offers a complete range portfolio of HLA testing assays. For more information, please feel free to contact us directly.

Reference

1. Lakhashe, S.K.; et al. Quantitation of HLA proteins incorporated by human immunodeficiency virus type 1 and assessment of neutralizing activity of anti-HLA antibodies. J Virol. 2008, 82(1): 428-434.