PBMC Hu-NSG Mice Model Development Service

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

In vivo models grafted with human immune cells are essential for CAR-T therapy, GvHD, and tumor immunity research. When humanizing mice, peripheral blood mononuclear cells (PBMC) are of particular interest because they can quickly be implanted in immunodeficient mice. The human-derived cells in humanized PBMC mice are mainly T cells, which are suitable for various studies that require T cell immune response. Meanwhile, T cells can also over-immunize the recipient mice, which can cause graft-versus-host disease (GvHD), and cause the mice to die after a few weeks. Therefore, the humanized PBMC (hu-PBMC) mouse model is very suitable for short-term studies that require powerful effectors and memory T cells and NK cell functions.

The PBMC Hu-NSG mouse model from Creative Biolabs is a research-ready mouse model that can be provided upon request to be shipped to your institution or to participate in custom drug efficacy studies performed by our CAR-T preclinical in vivo evaluation scientists.

Brief Introduction

Humanized PBMC NSG mice models are used as in vivo models to study and evaluate tumor immunity, cell therapy, and GvHD. This model uses adult peripheral blood mononuclear cells with the fastest engraftment rate and supports short-term in vivo experiments that require mature T cell function. Created with adult peripheral blood mononuclear cells, it can be quickly engrafted and allows short-term studies that require mature human T cells. However, humanized PBMC mice show a low level of human B cells with no de novo immune response. The low B cell level is due to the decrease in the proportion of B cells over time, reflecting the strong expansion of T cells. In general, the humanized PBMC model can target T cell functions (such as immune checkpoint inhibitors/agonists, CAR-T cell therapy) to gain insight into human tumor immunology and therapeutic efficacy.

	CD34＋ HSC Hu-NSG Mice Model	PBMC Hu-NSG Mice Model
THERAPEUTIC AREAS	Infectious Diseases Immuno-oncology Immunology and more…	Graft vs. Host Disease (Immunology) Immuno-oncology Infection Disease and more…
BENEFITS	Long-term lifespan A functional immune system No donor cell immune reactivity towards host	Enables short to mid-term studies requiring human T cells Strong effector and memory T cell function T cell-driven GvHD
CELL DEVELOPMENT	T cells (MHC-restricted CD4 and CD8) Monocytes Macrophages Mast cells Myeloid (SGM3) NK cells (IL15) Dendritic cells	B cells T cells (human donor HLA-restricted CD4 and CD8)

PBMC Hu-NSG Humanization Process

Inject human peripheral blood mononuclear cells (hu-PBMC) into female NSG mice. The mice can be shipped to customers within a few days after implantation. Implantation (mainly T cells) will increase over time but is limited by the development of graft-versus-host disease.

Engraftment of PBMC into NSG mice.

Fig.1 Engraftment of PBMC into NSG mice.

Hu-CD34 NSG & PDX Capabilities

Our NSG mouse models have a higher degree of immunodeficiency. It lacks NK cells while lacking T cells and B cells, and innate immunity is compromised. The NSG model is very suitable for human tumor cell transplantation (CDX), human tumor tissue transplantation (PDX) for immune reconstruction.

Highlights

Convenience - pre-injected PBMC has been pre-injected and tested to be effectively implanted in NCG mouse models.
Efficient - Pre-screened PBMC saves time, labor, and costs related to donor qualification. There is no license requirement.
Quality - PBMC cell stock has been screened to observe its implantation rate, weight loss, and study period. The number of cells has been optimized and can be used in NCG mouse models.
Reliable - the engrafted NCG mouse is the result of the collaboration between industry leaders who have more than 100 years of comprehensive experience in providing high-quality animal models and human biologics for research.

Establishment of PBMC hu-NSG PDX model.

Fig.2 Establishment of PBMC hu-NSG PDX model.

Applications

PBMC Hu-NSG is an ideal choice for long-term in vivo research in the field of immunology and cell therapy. Specifically, they can be used to evaluate the CAR-T therapies including but not limited to: monitor CAR-T distribution in vivo, determine CAR-T persistency, and monitor Cytokine Release Syndromes.

For more detailed information, please feel free to contact us or directly sent us an inquiry.