Anti-PSCA (1G8) h(41BB-CD3ζ) CAR, pCDCAR1 vector

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Details

Target
PSCA

Targeting Cell Type
T Cell

Targeting Diseases
Non-small Cell Lung Cancer

Generation
Second

Vector Name
pCDCAR1

Vector Length
~8kb

Vector Type
Lentiviral vector

Receptor Construction
scFv-41BB-CD3ζ

Discription of Signaling Cassetes
41BB
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. On the basis of preclinical observation, this molecule can promote the persistence of antigen-specific and antigen-nonspecific chimeric antigen receptor T-cells to significantly increases antitumor activity.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta, which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.

Target

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Published Data

CAR scFv data FCM

Fig.1 After treatment with PBS or 1G8 (20 Ag/mL) for 24 hours, cells were stained with Annexin V and PI and analyzed by FACS.

CAR Construction :

Fig.1 After treatment with PBS or 1G8 (20 Ag/mL) for 24 hours, cells were stained with Annexin V and PI and analyzed by FACS.

1G8 induces cell death.

Gu, Z., Yamashiro, J., Kono, E., & Reiter, R. E. (2005). Anti–prostate stem cell antigen monoclonal antibody 1G8 induces cell death in vitro and inhibits tumor growth in vivo via a Fc-independent mechanism. Cancer research, 65(20), 9495-9500.

Complete CAR data FCM

Fig.2 Generation of anti-prostate stem cell antigen (PSCA) CAR-T cells.

CAR Construction : 1G8 scFv-28ζ

Fig.2 Generation of anti-prostate stem cell antigen (PSCA) CAR-T cells.

Representative flow cytometric analyses of transfected T cells detected by flow cytometry.

Wu, D., Lv, J., Zhao, R., Wu, Z., Zheng, D., Shi, J., ... & Yao, Y. (2020). PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomarker Research, 8(1), 1-11.

Complete CAR data Cyt

Fig.3 Anti-PSCA CAR-T cells exhibited dramatic antitumor efficacy ex vivo.

CAR Construction : 1G8 scFv-28ζ

Fig.3 Anti-PSCA CAR-T cells exhibited dramatic antitumor efficacy ex vivo.

The lytic capacity towards MKN-28 cells, was analyzed at the indicated effector-to-target ratios in a 24 h lysis assay.

Wu, D., Lv, J., Zhao, R., Wu, Z., Zheng, D., Shi, J., ... & Yao, Y. (2020). PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomarker Research, 8(1), 1-11.

Complete CAR data FuncS

Fig.4 Anti-PSCA CAR-T cells efficiently reduced tumor progression in BGC-823 models.

CAR Construction : 1G8 scFv-28ζ

Fig.4 Anti-PSCA CAR-T cells efficiently reduced tumor progression in BGC-823 models.

Percentage of T cells in the spleen of the BGC-823 models and representative FACS plots of the i.v group and the p.t group, respectively.

Wu, D., Lv, J., Zhao, R., Wu, Z., Zheng, D., Shi, J., ... & Yao, Y. (2020). PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomarker Research, 8(1), 1-11.

Complete CAR data Inhibit

Fig.5 Anti-PSCA CAR-T cells suppressed tumor progression in MKN-28 model.

CAR Construction : 1G8 scFv-28ζ

Fig.5 Anti-PSCA CAR-T cells suppressed tumor progression in MKN-28 model.

Schematic representation depicting the time course of the experiment.

Wu, D., Lv, J., Zhao, R., Wu, Z., Zheng, D., Shi, J., ... & Yao, Y. (2020). PSCA is a target of chimeric antigen receptor T cells in gastric cancer. Biomarker Research, 8(1), 1-11.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.